植物乳杆菌SC2胞外多糖合成条件的研究  

Research on Optimization of Exopolysaccharide Production by Lactobacillus casei subsp, rhamnosus SC2

作　　者：张天琪[1,2] 张雪 李达 孔保华[1] 杨贞耐 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]中国农业科技东北创新中心农产品加工研究中心,吉林长春130033

出　　处：《农产食品科技》2011年第1期14-18,共5页

基　　金：国家自然科学基金面上项目（30670057）、农业部现代农业产业技术体系建设专项资金资助项目（ny-eytx-0502）.

摘　　要：从酸菜中筛选出产胞外多糖优良菌株植物乳杆菌SC2，对其培养基组成和培养条件进行研究，确定菌株SC2代谢产生胞外多糖的最适条件为：MRS培养基中含有麦芽糖30g/L、蛋白胨15g/L，初始pH值6．0，于30℃厌氧培养16h。优化后菌株SC2的胞外多糖产量可达到274mg／L，是优化前产量的5倍。The culture broth compositions and culture conditions of exopolysaccharide-producing Lactobacillus plantarum isolated from Northeast sauerkraut were studied. The optimal condition for producing exopolysacchride was determined that the carbohydrate source was maltose 30 g/L, the nitrogen source was peptone 15 g/L, the initial pH of culture medium was 6.0, and the strain SC2 was cultivated at 30℃ for 16 h. Under above culture conditions, the strain SC2 produced exopolysaccharide 274 mg/L, 5 fold than the treatment without optimization.

关 键 词：植物乳杆菌SC2 胞外多糖 培养条件优化 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]