产酯酵母和耐高温酵母原生质体形成与再生条件研究  

Studies on protoplast formation and regeneration of ester-producing yeast and thermotolerant yeast

作　　者：方尚玲[1] 刘源才 郭圣祥 李锐利[1] 陈茂彬[1] 

机构地区：[1]发酵工程省部共建教育部重点实验室湖北工业大学生物工程学院,湖北武汉430068 [2]劲牌有限公司,湖北大冶435100

出　　处：《酿酒》2011年第2期44-46,共3页Liquor Making

基　　金：国家自然科学基金项目(31071594);湖北省自然科学基金(2008CDB063);2009年博士科研启动基金BSQD0910;发酵工程省部共建教育部重点实验室开放基金(2009KFJJ01)

摘　　要：选取脱壁预处理时间、蜗牛酶浓度、酶解时间三因素设计三因素三水平正交试验来研究产酯酵母和耐高温酵母原生质体形成与再生条件。结果表明,产酯酵母原生质体形成与再生最佳条件为:预处理20min、1%蜗牛酶酶解30min;耐高温酵母为:预处理10min,1.5%蜗牛酶酶解60min。Through the three factors（includeing pretreatment time,enzyme concentra-tion,enzymolysis time）three levels orthogonal test to study the optimal conditions of protoplast formation and regeneration of ester-producing yeast and thermotolerant yeast.The result indicated that the optimal conditions of protoplast formation and regeneration for ester-producing yeast were as follows：pretreatment was 20 minutes,snail enzyme concentration was 1%,zymolysis time was 30 minutes;for thermotoler-ant yeast were that pretreatment was 10 minutes,snail enzyme concentration was 1.5%,zymolysis time was 60 minutes.

关 键 词：产酯酵母 耐高温酵母 原生质体形成与再生 正交试验 

分 类 号：TS261.11[轻工技术与工程—发酵工程] TS261.15[轻工技术与工程—食品科学与工程]