彩色小麦色素的光谱及抗氧化特性  

The Pigment Color Wheat Spectroscopic and Antioxidant Properties

作　　者：周桂娟[1] 王坤[1] 刘佳明[1] 孙慧君[1] 李集临[1] 张杰[1] 

机构地区：[1]哈尔滨师范大学,哈尔滨150025

出　　处：《中国农学通报》2011年第5期109-114,共6页Chinese Agricultural Science Bulletin

基　　金：哈师大博士科研启动基金项目"黑粒小麦色素的分子遗传研究"

摘　　要：国内外对彩色小麦色素作了一定的开发研究,但彩色小麦清除二苯基-2-苦肼基自由基的研究还很少。此文研究彩色籽粒色素的光谱及抗氧化特性,通过以普通小麦(中国春)和抗坏血酸为对照,用DPPH法测定黑粒小麦,蓝粒小麦,紫粒小麦,红粒小麦,白粒小麦色素的抗氧化、清除自由基的能力,其随着各种小麦色素浓度的提高,其抗氧化、清除自由基的能力也相应地提高。各种彩色小麦色素粗提物具有量效关系的抗氧化活性,其清除自由基能力大于普通小麦,但小于抗坏血酸,其中黑粒小麦色素清除自由基的能力最强。普通小麦、黑粒小麦、蓝粒小麦、紫粒小麦、红粒小麦、白粒小麦和Vc清除50%DPPH自由基的有效浓度(EC50)分别为188.27、54.26、76.22、94.05、126.24、110.04、0.139,其自由基清除能力AE分别为0.53×10-2、1.84×10-2、1.31×10-2、1.06×10-2、0.79×10-2、0.91×10-2、719×10-2。结果表明随着小麦种皮颜色的加深其色素粗提物清除自由基的能力就越强,这都为进一步研究、开发和利用彩色小麦的色素奠定理论基础。In color pigment made of wheat, but the research and development of wheat ErBenJi clear color-2-bitter hydrazine base of free radicals are rarely studied. Studies of the pigment color grain spectroscopic and antioxidant properties in common wheat, through and ascorbic acid and determination by DPPH black wheat, blue, violet wheat grain of wheat, red, white, wheat grain of wheat pigment antioxidant, scavenging free radicals, the ability of the wheat with the increase of the concentration of the pigment, its antioxidant ability of free radicals, correspondingly improved. All sorts of color pigment crude extractings wheat with concentration-response relationship of antioxidant activity, and its free radicals ability than common wheat, but less than ascorbic acid, including black wheat pigment scavenging free radicals, the strongest blue and purple wheat grain of wheat scavenging free radicals, white wheat ability and red wheat clear ability weak but most common wheat than. This explains the darker skins of wheat, the stronger ability of free radicals. Common wheat, black, blue wheat grain of wheat, red, purple wheat grain of wheat, white wheat and Vc DPPH 50% clear of free radicals EC 50 concentration （effective） respectively188.27, 54.26, 76.22, 94.05, 126.24, 110.04, 0.139. Its free radical scavenging capacity AE respectively by 0.53 × 10 -2 , 1.84 × 10 -2 , 1.31 × 10 -2 , 1.06 × 10 -2 , 0.79 × 10 -2 , 0.91 × 10 -2 , 719 × 10 -2 . Such as wheat color skin of a crude extractings pigment its ability of free radicals, which are better for further research, development and utilization of wheat color pigment theoretical basis.

关 键 词：色素 光谱 DPPH 自由基清除 抗氧化能力 

分 类 号：S512.9[农业科学—作物学]