优化的ATP生物发光法快速检测面粉中细菌总数  

Research on the Rapid Detection of Bacterial Count in Flour with Optimal ATP Bioluminescent Method

作　　者：李利霞[1] 伍金娥[1] 常超[1] 张佳艳[1] 王凌[1] 

机构地区：[1]武汉工业学院食品科学与工程学院,湖北武汉430023

出　　处：《安徽农业科学》2011年第7期4055-4057,共3页Journal of Anhui Agricultural Sciences

基　　金：湖北省教育厅项目(Q20081801);武汉工业学院创新基金项目(08cx006)

摘　　要：[目的]建立一种快速检测面粉中细菌总数的方法。[方法]采用优化的ATP生物发光法检测面粉中的细菌总数,考察D-荧光素(Ln)浓度、萤火虫荧光素酶(FL)浓度、Mg2+浓度对生物发光反应的影响。[结果]Ln浓度为70 mg/L,FL浓度为50 mg/L,Mg2+浓度为0.250 0 mmol/L时,ATP生物发光法的测定结果较理想。该法最低检测细菌总数为1.0×103cfu/ml,与传统的平板计数法具有良好的相关性(P>0.05),RSD为4.0%。[结论]优化的ATP生物发光法成本低、操作简便、反应迅速、准确可靠,能快速检测出食品中的细菌总数。[Objective] The rapid detection method of the bacterial count in flour was established.[Method] The bacterial count in flour was tested with optimal ATP bioluminescent method and the influence of the concentration of Ln,FL and Mg2＋ on ATP bioluminescence response was studied.[Result] The results indicated that when the concentration of Ln was 70 mg/L;FL,50 mg/L and Mg2＋,0.250 0 mmol/L,the determination result from the method was relatively perfect.The minimum of bacterial count（1.0×103 cfu/ml） could be detected with the method,which was correlated well with the method of conventional plate count（P〉0.05） and RSD was 4.0%,[Conclusion] This method was with characteristics of low cost,easy operation,fast response,accurate and reliable,which was capable for the rapid detection of bacterial count in food.

关 键 词：ATP生物发光法 平板计数法 面粉 细菌总数 

分 类 号：S37[农业科学—农产品加工]