重金属汞螯合物人工抗原的合成与鉴定  

Synthesis and Identification of Artificial Antigen for Mercury Chelate

作　　者：蒋芸[1] 严文静[2] 刘丽强[2] 匡华[2] 陈伟[2] 张坤[2] 胥传来[2] 

机构地区：[1]苏州大学分析测试中心,江苏苏州215123 [2]江南大学食品学院,江苏无锡214122

出　　处：《食品科学》2011年第7期244-246,共3页Food Science

摘　　要：以重金属汞为起始物质,将其离子化为二价汞,与双功能螯合剂谷胱甘肽(GSH)螯合后,获得半抗原,然后将半抗原与载体蛋白牛血清白蛋白(BSA)、卵清蛋白(OVA)进行偶联,获得完全抗原。实验对各步骤的反应条件进行优化,采用紫外光谱、凝胶电泳对产物进行表征,成功制备了汞-GSH-BSA完全抗原。为计算完全抗原中重金属和载体蛋白的偶联比,采用石墨炉原子吸收法测定产物中重金属汞的含量,采用二喹啉甲酸(BCA)法测定抗原中载体蛋白的含量,所制备的免疫原中汞和蛋白的偶联比是9:1。Hg-GSH-BSA的成功合成是制备可识别重金属汞螯合物抗体的基础。Mercury was used as the initial material to chelate GSH and obtain a bi-functional hepten of Hg-GSH chalete.The hapten was then coupled with the carrier protein such as bovine serum albumin（BSA） or ovalbumin（OVA） to obtain a complete antigen,Hg-GSH-BSA（OVA）,whose successful synthesis was confirmed by ultra-violet spectrum and SDS-PAGE.On the basis of mercury content in synthesized products determined by atomic absorption spectrometry and the amount of carrier protein determined by bicinchoninic acid（BCA） assay,the coupling ratio between mercury and carrier protein calculated to be 9：1.The successful synthesis of Hg-GSH-BSA conjugate may lay a basis for preparing recognizing antibodies of mercury chelate.

关 键 词：汞 重金属 免疫学检测 双功能螯合剂 抗原 

分 类 号：TS207.5[轻工技术与工程—食品科学]