红芝固态发酵产漆酶条件研究  

Research on Solid Fermentation of Ganoderma lucidum to Produce Laccase

作　　者：赵亮亮[1,2] 李燕荣[1] 傅宏兵[1] 叶红[1] 谌斌[2] 

机构地区：[1]江苏洋河酒厂股份有限公司,江苏宿迁223800 [2]广西环境工程与保护评价重点实验室,广西桂林541004

出　　处：《酿酒科技》2011年第4期25-28,共4页Liquor-Making Science & Technology

基　　金：广西环境工程与保护评价重点实验室基金资助项目(桂科能0702Z022);广西环境工程与保护评价重点实验室平台建设项目[桂科研(2008)27号]

摘　　要：对红芝固态发酵产漆酶的培养基和培养条件进行了研究。结果表明,适宜的固态发酵培养基为:以1.5∶1甘蔗渣和麦麸为基质,葡萄糖0.5%、硫酸铵5%、CuSO4.5H2O 0.01%、培养基含水率73%;适宜的固态培养条件为:初始pH值4.0,接种量20%(V∶W),28℃静置培养8 d,酶活达到最高23803 U/g干曲。The culture mediums and culture conditions of solid fermentation of G.lucidum to produce constitutive laccase were investigated.The optimum culture mediums were composed of substrate（the ratio of bagasse to wheat bran appropriate was 1.5∶1）,0.5 % glucose,5 % ammonium sulfate,0.01 % CuSO4·5H2O,and 73 % moisture content of substrate.The best culture conditions were summed up as follows： the initial pH value was 4.0,inoculating quantity was 20 %（V∶W）,and culture at 28 ℃for 8 d.The activity of the produced laccase could reach 23803 U/g dry starter eventually.

关 键 词：红芝 漆酶 固态发酵 

分 类 号：TQ925[轻工技术与工程—发酵工程]