香菇多糖径向流色谱分离工艺条件优化  

Optimization for Separation Conditions of Polysaccharides Using Radial Flow Ion-exchange from Lentinula edodes

作　　者：程俊文[1,2] 贺亮[1,2] 吴学谦[1,2] 付立忠[1,2] 胡传久[1,2] 吴庆其[1,2] 李海波[1,2] 魏海龙[1,2] 

机构地区：[1]浙江省林业科学研究院浙江省森林资源生物与化学利用重点实验室,杭州310023 [2]丽水市食用菌研究开发中心浙江益圣菌物发展有限公司,浙江丽水323000

出　　处：《中国林副特产》2011年第2期1-4,共4页Forest By-product and Speciality in China

基　　金：948项目"食药用菌活性多糖生物酶法提取与检测技术引进"(编号:2008-4-64);浙江省省院合作项目(编号:2008SY02);浙江省林业科技项目(07A07);浙江省创新团队建设与人才培养项目(2009F20019)

摘　　要：在单因素试验的基础上,通过Box-Benhnken组合设计和响应曲面分析法优化香菇多糖径向流色谱分离的工艺条件。响应面分析结果表明,当样品浓度为10mg/mL,上样量31mL,上样流速19mL/min,洗脱流速27mL/min,在次条件下多糖回收率达到87.33%,比传统轴向色谱分离及化学方法效率更高,时间更短。Radial flow chromatography（RFC） packed with ion-exchange resin was used to purify the Lentinula edodes crude polysaccharides.Based on the single factors of purification efficiency,response surface experiments（RSM） was employed to obtain the optimization.The purification efficiency of RFC was compared with that of axial column and traditional chemical method.RSM analysis showed good correspondence between experimental and predicted values.The optimal conditions for the purification of Lentinula edodes polysaccharides with RFC were determined： sample concentration of 10mg/mL,sample volume of 31mL,sample flow-rate of 19mL/min and elution flow-rate of 27mL/min.The recovery of polysaccharide is 87.33% under these conditions.The comparative test results indicated that the best purification efficiency was obtained by RFC within the shortest time.

关 键 词：香菇多糖 径向流色谱 响应面法 分离 

分 类 号：TQ536.1[化学工程—煤化学工程]