基于遗传算法的卵黄高磷蛋白提取工艺优化  

Optimized process of extracting phosvitin from hen egg yolk based on genetic algorithm

作　　者：徐彩娜[1] 殷涌光[1] 王二雷[2] 刘静波[2] 林松毅[2] 张刚[1] 

机构地区：[1]吉林大学生物与农业工程学院,长春130022 [2]吉林大学军需科技学院,长春130062

出　　处：《吉林大学学报（工学版）》2011年第3期876-881,共6页Journal of Jilin University:Engineering and Technology Edition

基　　金：'863'国家高技术研究发展计划项目(2007AA10Z329)

摘　　要：为了对提取鸡蛋黄中卵黄高磷蛋白的工艺参数进行优化,在单因素的基础上采用二次通用旋转组合设计试验;考察了有机溶剂比例(正己烷/乙醇)和用量对卵黄高磷蛋白提取效果的影响,并基于遗传算法优化出了提取卵黄高磷蛋白的最佳工艺参数;采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法(简称SDS-PAGE)测定卵黄高磷蛋白的分子量。结果表明:当有机溶剂比例(正己烷/乙醇)为3.4/1、有机溶剂用量为148mL时,卵黄高磷蛋白的纯度高达81.62%;SDS-PAGE凝胶电泳结果表明:提取出的卵黄高磷蛋白的分子质量在35kD左右。The quadratic general rotary unitized design was employed to optimize the parameters of extracting phosvitin from egg yolk proceeding after one-factor experimental design. The ratio of n- hexane to ethonal and their total dosages were investigated to evaluate the extraction rate of phosvitin. The optimized technology parameters of extracting phosvitin were obtained based on genetic algorithm. The SDS-polyacrylamide gel electrophoresis （SDS-PAGE） was used to determine the molecular weight （MW） of phosvitin. The results are obtained as follws： ratio of n-hexane to ethonal of 3.4/1 （V/V）, dosage of the total organic solvent of 148 mL, the purity of the phosvitin reached 81.62%. The results of SDS-PAGE indicate that the MW of phosvitin was 35 kD.

关 键 词：食品加工技术 卵黄高磷蛋白 二次通用旋转组合设计 遗传算法 十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 高效液相色谱 

分 类 号：TS253.1[轻工技术与工程—农产品加工及贮藏工程]