南美白对虾虾头主要自溶酶的分离纯化及鉴定  

Purification and Identification of Major Autoenzyme from the Head of White Shrimp(Penaeus vannamei)

作　　者：银凤[1] 周爱梅[1] 张祥刚[1] 曹庸[1] 刘欣[1] 陈永泉[1] 

机构地区：[1]华南农业大学食品学院

出　　处：《食品与发酵工业》2011年第3期23-26,共4页Food and Fermentation Industries

基　　金：广东省科技计划农业攻关项目(2007A020100006-9)

摘　　要：以水解试验为导向,采用硫酸铵分级沉淀、Sephadex G-100凝胶层析、DEAE-DE52阴离子交换柱层析等手段分离纯化南美白对虾虾头主要自溶酶,同时采用抑制剂试验及SDS-PAGE对纯化蛋白酶进行鉴定。结果表明,南美白对虾虾头主要自溶酶经过纯化后比活力高达2.2×106U/g,纯化倍数为96.90。丝氨酸蛋白酶抑制剂苯甲基磺酰氟(PMSF)对主要自溶酶的抑制率高达91.3%;SDS-PAGE显示该酶为单一电泳带,且在电泳中与标准胰蛋白酶移动距离相同,表明南美白对虾虾头内的主要自溶酶为胰蛋白酶。With hydrolysis test as an orientation, the major autoenzyme was purified from the head of white shrimp （Penaeus vannamei） by sulfate precipitation, gel filtration on Sephadex G-100 followed by ion-exchange chromatography DEAE-DE52. The autoenzyme was then identified by inhibitor tests and SDS-PAGE analysis. The results showed the major autoenzyme from the head of white shrimp had a specific activity of 2.2 × 10^6 U/g with a 96.90-fold increase over that of the crude enzyme extract. This autoenzyme was inhibited by serine protease inhibitor phenyl methyl sulfonyl fluoride （PMSF） with the inhibition rate being 91.3% , and illustrated as a single band in SDS-PAGE with the same distance as that of standard trypsin. These results indicated that trypsin was the major autoenzyme in the head of white shrimp.

关 键 词：南美白对虾 自溶酶 分离纯化 鉴定 

分 类 号：R151[医药卫生—营养与食品卫生学]