莱克多巴胺和土霉素人工抗原的合成及鉴定  

Synthesis and Identification of Oxytetracycline and Ractopamine Antigens

作　　者：宋珊珊[1] 彭池方[1] 匡华[1] 严文静[1] 孙萌萌[1] 胥传来[1] 

机构地区：[1]江南大学食品学院,江苏无锡214122

出　　处：《食品科学》2011年第9期167-169,共3页Food Science

摘　　要：研究莱克多巴胺和土霉素免疫抗原的制备。实验中用碳二亚胺法将莱克多巴胺(ractopamine,RAC)与载体蛋白牛血清白蛋白(bovine serum albumin,BSA)偶联,制备完全抗原;采用混合酸酐法将土霉素(oxytetracycline,OTC)与载体蛋白BSA偶联,制备完全抗原,并对合成产物进行表征。紫外扫描对合成产物RAC-BSA、OTC-BSA进行鉴定结果显示偶联成功,经重氮化后利用混合酸酐法制备完全抗原的偶联比为5:1;经衍生后利用碳二亚胺法制备RAC完全抗原偶联比为7:1。利用制备的免疫原免疫兔子可以获得相应的抗体。In order to explore the preparation of antigen for ractopamine（RAC） and oxytetracycline（OTC）,RAC and OTC were conjugated with the carrier protein BSA by ethyl carbodiimide hydrochloride（EDC） method and mixed anhydride method to achieve RAC-BSA and OTC-BSA,respectively.Both OTC-BSA and RAC-BSA were identified with UV-visible spectros-copy to reveal the successful coupling with a molar coupling ratio of 5：1 and 7：1,respectively.Polyclonal antibodies were prepared by immunizing New Zealand white rabbits with RAC-BSA and OTC-BSA.The obtained polyclonal antibodies can be used to detect RAC and OTC.

关 键 词：土霉素 莱克多巴胺 偶联 抗原 

分 类 号：TS202.3[轻工技术与工程—食品科学]