直接竞争化学发光酶免疫法检测猪肉中磺胺嘧啶  

Determination of Sulfadianzine Residues in Pork by Direct Competitive Chemiluminescence Enzyme Immunoassay

作　　者：楚金申[1] 许杨[1] 何庆华[1] 王刘花[1] 卢江[1] 

机构地区：[1]食品科学与技术国家苹点实验室 南昌大学中德联合研究院,江西南昌330047

出　　处：《食品科学》2011年第10期124-129,共6页Food Science

基　　金：江西省科技支撑计划项目(2007BS12501);国家“863”计划项目(2007AA10Z427)

摘　　要：建立一种检测猪肉中磺胺嘧啶的简便、快速、高特异性的直接竞争化学发光酶免疫法。采用改良的过碘酸钠法制备酶标抗体,以辣根过氧化物酶催化鲁米诺-过氧化氢作为化学发光体系。经优化该方法检测条件为:利用柠檬酸缓冲液稀释抗原包被质量浓度为0.8μg/mL,酶标抗体2000倍稀释,竞争反应25min;所建立的方法分析灵敏度为2.96ng/mL,批内变异系数小于8.32%,批间变异系数小于13.4%,以猪肉为样品的分析回收率为75.6%～103.7%,与其他结构类似物未见明显交叉,所建立的标准曲线相关系数为0.9922、检测线性范围为4.14～244ng/mL、检测时间为25min,可在实际生产中应用。A direct competitive chemiluminescence enzyme immunoassay（DC-CLEIA） was developed to determine sulfadi-azine（SD） residues in pork.Assay parameters including coating antigen（OVA-SD） and enzyme labeled antibody concentrations concentrations and reaction time were optimized to be 0.8 μg/mL,2000-fold dilution and 25 min,respectively.The analytical sensitivity of the developed method was 2.96 ng/mL,and the intra-assay and inter-assay coefficients of variation were below 8.32% and 13.4%,respectively.The recoveries for SD in negative pork spiked at three levels ranged from 75.6% to 103.7%.The McAb had no noticeable cross-reactivity to SD analogues.The standard curve displayed good linearity over the range of 4.14 to 244 ng/mL with a correlation coefficient of 0.9922.This DC-CLEIA may be used for routine detection and monitoring of SD in pork.

关 键 词：磺胺嘧啶 直接竞争化学发光酶免疫法 辣根过氧化物酶 猪肉 

分 类 号：TS207.3[轻工技术与工程—食品科学]