拟除虫菊酯类农药通用人工抗原的合成与鉴定  

Synthesis and Identification of Generic Antigen for Pyrethroid Insecticides

作　　者：王俊平[1] 余桂春[1] 刘冰[1] 杨丽[1] 王帅[1] 石曼[1] 王硕[1] 

机构地区：[1]天津科技大学食品营养与安全教育部重点实验室,天津300457

出　　处：《现代食品科技》2011年第5期524-527,共4页Modern Food Science and Technology

基　　金：国家"十一五"科技支撑计划项目(2009BADB9B03;2009BADB9B06)

摘　　要：根据拟除虫菊酯公共结构设计合成一种拟除虫菊酯通用半抗原,所有中间体及半抗原经电喷雾电离质谱(ESI-MS)、红外光谱(IR)和核磁共振(1H NMR)鉴定确定结构。通过活化酯法将通用半抗原与钥孔血蓝蛋白(KLH)偶联制备出免疫原,紫外吸收法鉴定表明免疫原偶联成功,研究为建立拟除虫菊酯多残留免疫分析方法奠定了基础。A generic hapten was synthesized based on the common structure of pyrethroid insecticides.All intermediates and hapten were verified by ESI-MS,IR,and 1H NMR spectrometry.By active ester method,the hapten was conjugated with KLH to prepare the immunogen.From the UV ansorption spectroscopy the conjugate were successfully prepared.It is a base for development of multi-residue immunoassay for pyrethroid insecticides detection.

关 键 词：拟除虫菊酯 通用人工抗原 合成 鉴定 

分 类 号：TQ453.2[化学工程—农药化工]