从衣霉素抗性突变体中筛选α-淀粉酶高产菌株  

HIGH PRODUCTION STRAIN OF α-AMYLASE FROM TUNICAMYCINRESISTANT MUTANTS OF B.SUBTILIS BF7658

作　　者：贾士芳[1] 郭兴华[1] 

机构地区：[1]中国科学院微生物研究所

出　　处：《微生物学报》1990年第1期83-85,共3页Acta Microbiologica Sinica

基　　金：国家科委资助项目

摘　　要：α-淀粉酶是产量大用途广的酶制剂之一。促使α-淀粉酶高产的基因是它的调节基因,而它的调节基因又有两种,一种是既可调控α-淀粉酶结构基因,又可调控蛋白酶结构基因以及其它酶结构基因;Tunicamycin-resistant genes were map-ped near the α-amylase structure gene (amyE)and regulation gene (amyR).The gene or-der around amyE is Lin2-tmrA7-amyR-amyE-tmrB8-aroI906.Tunicamycin-resistant mu-tants were isolated from B.subtilis BF 7658after treatment with NTG.They are ableto grow normally in a BPY mediumcontaining 50 μg of tunicamycin per ml of so-lution.The mutants were classified into twotypes by activity of extracellular α-amylase.One type which is about 28% in total mutantscan raise the activities of α-amylase.Butanother type which is about 72% in total mu-tants did not change the activities though theyare tunicamycin-resistant mutants.We have screened the high productionstrains which raiseed the activity of α-amyla-se about 50% from B.subtilis BF7658.

关 键 词：枯草杆菌 Α-淀粉酶 高产菌株 筛 

分 类 号：TQ925.1[轻工技术与工程—发酵工程]