嗜酸乳杆菌NCFM粘附宿主后对其粘附相关基因表达的影响  

Lactobacillus acidophilus NCFM affects host adhesionrelated gene expression after adhering to host

作　　者：韩琳琳[1] 满朝新[2] 吕学娜[1] 王明娜[1] 张光辉[1] 刘颖[1] 姜毓君[1,2] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室,哈尔滨150030 [2]东北农业大学国家乳业工程技术研究中心,哈尔滨150030

出　　处：《微生物学报》2011年第5期609-614,共6页Acta Microbiologica Sinica

基　　金：长江学者和创新团队发展计划(IRT-0959-203);国家科技支撑计划项目(2009BADB9B06);黑龙江省博士后科研启动资助金(LBH-Q07023);东北农业大学创新团队项目(CXT007-3-2)~~

摘　　要：【目的】益生菌粘附于肠道上皮细胞上是它的一种益生作用。本研究通过体内外实验,分析嗜酸乳杆菌NCFM对粘附相关基因的影响。【方法】利用GO(Gene Ontolog)分类筛选Human Genome U133 Plus 2.0Array基因表达谱芯片中的粘附相关基因,通过体外Caco-2细胞培养模型和体内小鼠粘附模型,采用Real-time PCR方法对粘附相关基因进行验证分析。【结果】经NCFM作用后,12个粘附相关基因呈上调表达。利用Real-time PCR验证,12个基因在体内和体外经嗜酸乳杆菌NCFM作用后亦均同样为上调表达,其中CCL2基因上调表达最为明显。【结论】经体内外研究表明,嗜酸乳杆菌NCFM粘附肠上皮细胞后能够引起宿主粘附相关基因出现特定表达变化,为今后深入揭示其粘附作用提供必要基础。[Objective]Adhering to the intestinal epithelial cells is one of the beneficial functions exerting by probiotics.We tested and verified the expression of adhesion-related genes in vivo and in vitro to understand the influence from Lactobacillus acidophilus NCFM on host cells.[Method]We selected the adhesion-related genes through GO（gene ontology） category from the Human Genome U133 Plus 2.0 Array analysis.These genes were verified by in vitro Caco-2 cells culture model and in vivo mouse model using Real-time PCR method.[Results] After L.acidophilus NCFM adhering to Caco-2,we found that 12 adhesion-related genes were up-regulated.The up-regulation was confirmed in vivo and in vitro by Real-time PCR assay.Among them,the up-regulated expression of chemokine（C-C motif） ligand 2（CCL2） gene was the most distinguished one.[Conclusion]Our research showed that L.acidophilus NCFM,adhering to Caco-2 cells,could cause the differential expression of the host adhesion-related genes.Results of this study seemed to provide some useful data for further revealing its effect on intestinal epithelial cells.

关 键 词：嗜酸乳杆菌NCFM CACO-2细胞 粘附 基因表达 

分 类 号：Q93[生物学—微生物学]