分叉双歧杆菌在固态发酵体系中的产酸特性  

作　　者：韩润林[1] 张建安[1] 张小勇[1] 李佐虎[1] 李立民[2] 赵智宠 

机构地区：[1]中科院化工冶金研究所生化工程国家重点实验室,北京100080 [2]内蒙古农牧学院生物工程系,呼和浩特010018

出　　处：《食品科学》1999年第8期28-32,共5页Food Science

摘　　要：以液态发酵（SmF）为参照体系，对分叉双歧杆菌（B．bifidum）在固态发酵（SSF）体系（培养基含水量为54．5％和68.8％）中的生长特性进行了研究。结果表明：1、在SSF体系中B．bifidum的产酸特性与SmF体系有显著的差异，在SSF中既产生乳酸、乙酸也产生丙酸和丁酸，在SmF中却不产生丙酸和丁酸，可以推测在肠道中，B．bifidum有可能分泌丙酸和丁酸以实现其特殊的生理作用，如调节肠道群或抵御突变等。两体系中乳酸和乙酸的摩尔比分别为1.26～1．62．：1（SSF）和1：2（SmF），SSF体系的pH下降速度小于SmF体系；2、在发酵12h时,SSF体系（培养基含水量54．5％）中B．bifidum膜脂肪酸组成与SmF体系有显著差异，主要表现为软脂酸和油酸增加（分别增加8．3％和41．2％），硬脂酸减少26．9％，但亚油酸不变，这可能有利于适应环境；3.B．bifidum在SSF体系中生长较缓慢，最大活菌数小于SmF体系，但对数期、衰亡期延长。B.hifidunlis one species of bifidobacteria living in the human large intestine.The in vitro studies on B.bifidum are usually performed in submerged fermentation (SmF) system.xt.hich is not true real gut environment.In this patper the growth characteristics of B.bifidum in solid state fermentation (SSF) system (water content of media.54.5% and 68.8%).compared ti,ith SmF were studied.The results show:(1) Besides lactic acid(LA)and acetic acid (AA) B.billdum in SSF secretes propionic acid (PA) and butyric acid (BA).however it only produces LA and AA in SmF .It is assumde that in human large intestine B.bifidum can secretes PA and BA. which appear to be beneficial ic regulate microeubiosis and to improve the human health. The ratio of LA to AA in SSF (1 .26 ~ 1 .62: 1) differs from that in SmF(1 .2) .(2) In SSF cell membrane fatty acids measured at the twelfth hour of fermentation show increased levels of C 16: 0 and C 18: 1 and decreased level of C 18:0 These changes may be related to the production of PA and BA .(3)There are a lower growth rapid.a less viable count longer logarithmic growth phase and death phase for B.bifidum cultivated in SSF than those in SmF.

关 键 词：双歧杆菌 生理功能 分叉双歧杆菌 固态发酵 

分 类 号：TS201.3[轻工技术与工程—食品科学]