酵母β-葡聚糖亚慢性毒性实验研究  

Subchronic toxicity of yeast β-glucan

作　　者：张馨[1] 方瑾[1] 王伟[1] 崔文明[1] 贾旭东[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100021

出　　处：《中国食品卫生杂志》2011年第3期236-238,共3页Chinese Journal of Food Hygiene

摘　　要：目的研究酵母β-葡聚糖的亚慢性毒性,提出其未观察到有害作用水平(NOAEL)。方法 80只断乳SD大鼠按体重随机分成4组,即对照组和受试物3个剂量组,每组20只,雌雄各半。低、中、高剂量分别为0.62、1.25、2.50 g/kg BW。各剂量组灌胃给予受试物,对照组灌同体积的蒸馏水。动物单笼喂养,自由饮食,每周记录大鼠进食量,每周称2次体重以调整灌胃量,连续观察90 d,于实验中期(45 d)和实验结束采血测各项血液学和临床生化指标,并进行病理组织学检查。结果受试物各剂量组动物体重、进食量、食物利用率、血液学检测指标、临床生化指标、各脏器脏体比与对照组比较,差异均无统计学意义;病理指标也未显示由受试物引起的异常改变。结论根据本次实验结果确定酵母β-葡聚糖NOAEL在雌雄大鼠均为2.50 g/kg BW,即本实验的高剂量,相当于人体推荐量的600倍。Objective To investigate the subchronic toxicity of yeast β-glucan and to determine its no-observed-adverse-effect-level（NOAEL）.MethodsEighty Sprague-Dawley rats were randomly divided into four groups with twenty rats（10 males and 10 females） in each group.Four groups of animals were treated respectively with 0,0.62,1.25,and 2.50 g/kg BW of test material by gavage for 90 days.Clinical observations were conducted daily.Body weights and food consumption were recorded weekly.Blood samples were collected from all rats on the 45th day and the day by the end of the study.Gross and histopathologic examinations and organ weights were performed on all animals euthanized for necropsy.Results All parameters including body weight,food consumption,hematology,clinical chemistry,absolute and relative organ weights and histopothological examination in treatment groups were comparable with the control group.ConclusionThe NOAEL of yeast β-glucan was 2.50 g/kg BW in male and female rats,which was the highest dose tested in this study and equivalent to 600 times of the quantiy of yeast β-glucan recommended for human use.

关 键 词：酵母Β-葡聚糖 亚慢性毒性 未观察到有害作用水平 

分 类 号：R114[医药卫生—卫生毒理学]