Effects of Er^(3+) on the proliferation,differentiation and mineralization function of primary mouse osteoblasts in vitro  

作　　者：张金超 孙静 张大威 李亚平 郝晓红 秦新英 

机构地区：[1]Chemical Biology Key Laboratory of Hebei Province,College of Chemistry and Environmental Science,Hebei University [2]Affiliated Hospital of Hebei University [3]Department of Natural Product Chemistry,Shenyang Pharmaceutical University

出　　处：《Journal of Rare Earths》2011年第5期507-510,共4页稀土学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (20971034);Foundation for Key Program of Ministry of Education of China (208018);Returned Scholars of Hebei Province (207041);Nature Science Key Foundation of Hebei Province (B2009000161);Natural Science Foundation of Hebei University

摘　　要：A series of experimental methods including 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H tetrazolium bromide （MTT） test,alkaline phosphatase （ALP） activity measurement,oil red O stain and measurement,mineralized function and quantitive real time RT-PCR （qRT-PCR） were employed to assess the effects of Er3＋ on the proliferation,differentiation and mineralization function of primary osteoblasts （OBs） in vitro at cell and molecular levels. The results indicated that Er3＋ inhibited the proliferation of OBs at a concentration of 1×10–7 mol/L,but had no effect at other concentrations. Er3＋ inhibited the differentiation of OBs at concentrations of 1×10–8,1×10–7,and 1×10–6 mol/L,but had no effect at a higher concentration of 1×10–5 mol/L. Er3＋ had no effect on the transdifferentiation of OBs at tested concentrations. Er3＋ inhibited the mineralization function of OBs at concentrations of 1×10–7,1×10–6,and 1×10–5 mol/L,but had no effect at a lower concentration of 1×10–8 mol/L. The expression of the mRNA for runt-related transcription factor 2 （RUNX-2） and peroxisome proliferators activated receptor γ （PPAR-γ） was down-regulated in the presence of 1×10–6 mol/L Er3＋. These findings suggested that Er3＋ might have negative effect on bone metabolism.A series of experimental methods including 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H tetrazolium bromide （MTT） test,alkaline phosphatase （ALP） activity measurement,oil red O stain and measurement,mineralized function and quantitive real time RT-PCR （qRT-PCR） were employed to assess the effects of Er3＋ on the proliferation,differentiation and mineralization function of primary osteoblasts （OBs） in vitro at cell and molecular levels. The results indicated that Er3＋ inhibited the proliferation of OBs at a concentration of 1×10–7 mol/L,but had no effect at other concentrations. Er3＋ inhibited the differentiation of OBs at concentrations of 1×10–8,1×10–7,and 1×10–6 mol/L,but had no effect at a higher concentration of 1×10–5 mol/L. Er3＋ had no effect on the transdifferentiation of OBs at tested concentrations. Er3＋ inhibited the mineralization function of OBs at concentrations of 1×10–7,1×10–6,and 1×10–5 mol/L,but had no effect at a lower concentration of 1×10–8 mol/L. The expression of the mRNA for runt-related transcription factor 2 （RUNX-2） and peroxisome proliferators activated receptor γ （PPAR-γ） was down-regulated in the presence of 1×10–6 mol/L Er3＋. These findings suggested that Er3＋ might have negative effect on bone metabolism.

关 键 词：erbium ion OSTEOBLASTS PROLIFERATION DIFFERENTIATION MINERALIZATION rare earths 

分 类 号：R580[医药卫生—内分泌]