肝癌VHL基因突变的研究  

Research of VHL gene mutation in hepatocellular carcinoma

作　　者：刘凤军[1] 孙栋[1] 曲辉[1] 王可新[1] 何庆泗[1] 孙学英[1] 寿楠海[1] 

机构地区：[1]山东大学齐鲁医院普外科,山东济南250012

出　　处：《中国现代普通外科进展》2011年第3期169-172,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:研究肝癌VHL基因突变以及其与血管内皮生长因子、微血管密度等临床病理特征之间的关系。方法:取30例肝癌标本;设计一对引物覆盖VHL第二外显子,RT-PCR加DNA序列测定分析其中10例VHL基因,分析HCC与VHL突变的关系,比较有无VHL基因突变的HCC其VEGF、MVD及临床病理特征的差异。结果:10例HCC标本,3例发生VHL基因突变(30%);与未发生VHL基因突变7例HCC相比,VHL突变肝癌VEGF、MVD均增高(P<0.05);临床病理分析显示VHL基因突变HCC病理分级较高(P<0.05)。结论:肝癌VHL突变率约30%;其突变与其临床病理特点有关,VHL突变肝癌有较高的恶性和侵袭性。Objective： To explored the VHL gene mutation in HCC and its relations with VEGF,MVD and clinic-pathological features.Methods： 10 cases of HCC sterile tissue was obtained to analyse VHL gene mutation by RT-PCR and DNA sequenses analysis.A couple of primier which cover VHL exon 2 was designed by GeneBank.Immunohistochemistry methods was usde to assay VEGF and MVD.Results： Three of 10 cases of HCC were deteced VHL gene mutation（30%）.Compared with HCC without VHL mutation（7 cases）,HCC with VHL mutation（3 cases） have higher VEGF and MVD manifestation,and corresponding higher clinic-pathological grades（P≤0.05）.Conclusion： The carcinogenesis of HCC has close relation with VHL gene mutation.HCC has more than 30% VHL mutation.Compared with HCC without VHL mutation,HCC with VHL mutation usually represent higher invisive carcinoma.

关 键 词：VHL基因 肝癌 基因突变 血管内皮生长因子 微血管密度 

分 类 号：R735.8[医药卫生—肿瘤]