Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS  

作　　者：LI Guo-chen WANG Yan-hong WU Ren-an WANG Shi-cheng 

机构地区：[1]Inspection and Testing Center of Agro-product Safety and Environment Quality,Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,P.R.China [2]National Chromatographic Research&Analysis Center,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,P.R.China

出　　处：《Chemical Research in Chinese Universities》2011年第3期371-374,共4页高等学校化学研究(英文版)

基　　金：Supported by the Knowledge Innovation Program of the Chinese Academy of Sciences(No.KSCX2-YW-N-035);Projects of Shenyang City Science and Technology Burea, China(Nos.F10-224-4-00, 1091239-6-00)

摘　　要：The interest in the analysis of phospholipids(PLs), especially phosphatidylcholine(PC), has been increasing due to the importance of them in biochemistry as well as in industry. A method was reported based on the offline combination of MALDI-TOF MS and normal-phase HPLC for analyzing PLs extracted from crucian carp. Total PLs of crucian carp were extracted and then separated by HPLC before the collected subfractions were analyzed by MALDI-TOF MS. The mass spectra obtained show peaks of H+, Na+ and K+ adducts of PC molecules. It is shown that the prior separation of PLs by HPLC is highly necessary to remove the signal suppressing and to avoid the possible overlapping. With this method, 9 possible PC molecules in crucian carp and the corresponding fatty acid compositions were given from the well-resolved mass spectra.The interest in the analysis of phospholipids(PLs), especially phosphatidylcholine(PC), has been increasing due to the importance of them in biochemistry as well as in industry. A method was reported based on the offline combination of MALDI-TOF MS and normal-phase HPLC for analyzing PLs extracted from crucian carp. Total PLs of crucian carp were extracted and then separated by HPLC before the collected subfractions were analyzed by MALDI-TOF MS. The mass spectra obtained show peaks of H+, Na+ and K+ adducts of PC molecules. It is shown that the prior separation of PLs by HPLC is highly necessary to remove the signal suppressing and to avoid the possible overlapping. With this method, 9 possible PC molecules in crucian carp and the corresponding fatty acid compositions were given from the well-resolved mass spectra.

关 键 词：PHOSPHOLIPID PHOSPHATIDYLCHOLINE PHOSPHATIDYLETHANOLAMINE SPHINGOMYELIN HPLC MALDI Crucian Carp 

分 类 号：S917.4[农业科学—水产科学]