超声波辅助提取茶多酚工艺条件的优化  

Optimizing of ultrasonic wave-assisted extraction process of tea-polyphenols substance from green tea

作　　者：宋传奎[1] 肖斌[1] 王艳丽[1] 李瑞[1] 宋红霞[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2011年第5期133-139,146,共8页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家茶叶产业技术体系项目(Z225020801)

摘　　要：【目的】探索超声波辅助提取茶多酚的最佳工艺条件,为茶多酚的提取和综合利用奠定基础。【方法】以紫阳群体种绿茶为材料,采用二次回归正交旋转组合设计对茶多酚超声波辅助提取工艺进行优化。【结果】建立了料(g)液(mL)比、乙醇体积分数、超声时间、超声温度、超声波功率5因素与茶多酚提取率之间的回归优化模型,模型拟合检验P<0.000 1,决定系数R2=0.926 0,失拟性检验P=0.223 4>0.05不显著,模型达到极显著水平,无失拟因素存在,回归模型与实测值能够较好拟合。5因素对茶多酚提取率的影响大小依次为:料液比>乙醇体积分数>超声温度>超声时间>超声波功率。从模型得出的最佳提取工艺为:料(g)液(mL)比1∶35.8,乙醇体积分数77.6%,超声时间37.6 min,超声温度72.1℃,超声波功率248.4 W。【结论】优化了茶多酚超声波辅助提取的最佳工艺,在优化后的最佳工艺参数下,茶多酚提取率最高可达21.78%,较传统乙醇浸提方法提取时间缩短,且提取率提高了8.56%。【Objective】 The present study aimed to optimize ultrasonic wave-assisted extraction process of tea-polyphenols substance to provide theoretical parameters for its exploiture extraction and comprehensive utilization.【Method】 With Ziyang population variety as test material,quadratic orthogonal rotation combination design was used to optimize ultrasonic wave-assisted extraction process of tea-polyphenols substance.【Result】 A quadratic regression orthogonal model was established to express the relationship between total polyphenol yield and five factors including ratio of solid to liquid,ethanol concentration,ultrasonic time,ultrasonic temperature and ultrasonic Power;Equation fit test P0.000 1,coefficient of determination R2 = 0.926 0,loss of fit test P=0.223 40.05 not significant,the model was extremely significant,and there was no loss of fit factors,regression models with the measured values can be well fitted.The factor order on extraction yield from strong to weak was ratio of solid to liquidethanol concentrationultrasonic temperatureultrasonic timeultrasonic Power.According to this model,the best extraction technology is based on ultrasonic extraction at 72.1 ℃,248.4 W for 37.6 min using 77.6% ethanol and 1∶35.8（g/mL） of green tea and liquid.【Conclusion】 The optimal ultrasonic wave-assisted extraction conditions were determined.On the conditions the highest yield of tea-polyphenols was 21.78%.Compared to the traditional organic solvent extraction method at the same condition,the yield of TP was enhanced by 8.56% and the extraction time was reduced.

关 键 词：茶多酚 提取率 超声波辅助提取 工艺优化 

分 类 号：S571.1[农业科学—茶叶生产加工]