玉米芯固定化单宁酶的工艺条件优化  

Optimization of Process Conditions for Immobilizing Tannase by Corncob

作　　者：范超[1] 黎继烈[1] 王挥[1] 郝聚喜[1] 丁丽霞[1] 李忠海[1] 

机构地区：[1]中南林业科技大学生命科学与技术学院,湖南长沙410004

出　　处：《食品科学》2011年第11期123-128,共6页Food Science

基　　金：国家林业局"948"项目(2010-4-22);"十一五"国家科技支撑计划项目(2009BADB1B10)

摘　　要：探讨改性玉米芯固定化单宁酶的工艺条件。采用Box-Behnkens试验设计和响应面分析方法,对改性玉米芯固定化单宁酶的条件进行优化,得出其最佳工艺条件为:单宁酶酶液与玉米芯载体比26:1(V/m)、pH 6.8、时间8.0h、温度36℃,在此条件下固定化酶活力达到(16085±5)U/g,酶活力回收率为44.68%。对最佳条件下制备的固定化单宁酶的酶学特性进行研究,发现该固定化酶的最适反应pH值为4.5～5,最适反应温度为60℃。The process conditions for immobilizing tannase by modified corncob were explored.The Box-Behnken design and response surface methodology were used to optimize immobilization conditions of tannase by modified corncob.The results showed the optimal process conditions were enzyme-to-carrier ratio of 26：1,pH 6.8,immobilization time of 8.0 h and immobilization temperature of 36 ℃.Under the optimal conditions,the activity of immobilized enzyme was（16085 ± 5） U/g,the recovery rate of tannase activity was 44.68%.The optimal reaction pH and temperature of immobilized tannase were 4.5-5 and 60 ℃,respectively.

关 键 词：固定化单宁酶 改性玉米芯 BOX-BEHNKEN试验设计 响应面法 

分 类 号：TS201.21[轻工技术与工程—食品科学]