高产油脂红酵母的选育及培养条件优化  

Screening of Lipid-producing Rhodotorula and Optimization of Its Fermentation Conditions

作　　者：徐振杰[1] 胡容[1] 李珍[1] 程子彰[1] 黄乾明[1] 

机构地区：[1]四川农业大学生命科学与理学院,四川雅安625014

出　　处：《食品科学》2011年第11期209-215,共7页Food Science

基　　金：四川省教育厅重点科研项目(08ZA053);四川农业大学科技创新基金项目(00731200)

摘　　要：以本实验室早期从自然界中分离纯化所得18株高产类胡萝卜素红酵母菌株为材料,采用苏丹黑染色法初筛、摇瓶法复筛,得到1株脂含量相对较高(14.63%)的菌株SCAU-13;经鉴定,该菌株为禾本红酵母(Rhodotorulagraminis);以SCAU-13为出发菌株,经紫外诱变、硫酸二乙酯诱变和紫外-硫酸二乙酯复合诱变,最终得到突变菌株M124,其脂含量达42.42%,为出发菌株的2.90倍;培养条件优化结果表明其最佳产脂条件为:以葡萄糖为碳源,(NH4)2SO4为氮源,碳氮比为60:1,起始pH6.0,蛋白胨1.8g/L,酵母粉4g/L,MgSO4.7H2O 0.5g/L,在此条件下,菌体生物量为(13.55±0.10)g/L,脂含量为(53.67±0.17)%,产脂量为(7.27±0.03)g/L。Eighteen Rhodotorula strains（SCAU 1 through 18） that can produce carotenoids were isolated from natural environment and subjected to preliminary screening with Sudan Black dye and further screening by shake-flask culture.As a result,strain SCAU-13 was found to have the highest lipid content of 14.63% and identified as Rhodotorula graminis.The strain was mutagenized respectively with UV light,diethyl sulfate（DES） and their combination,resulting in 13 mutants,and strain M124 containing the largest amount of lipids among the 13 mutants was screened out,which was 42.42% and 2.90 times as high as that of the original strain SCAU-13.Orthogonal array optimization showed that the optimal fermentation conditions for lipid production by strain M124 were glucose as carbon source,（NH4）2SO4 as nitrogen source,carbon-to-nitrogen ratio of 60：1,initial pH 6.0,peptone concentration of 1.8 g/L,yeast extract concentration of 4 g/L and MgSO4·7H2O concentration of 0.5 g/L.Under the optimal culture conditions,the cellular biomass and the lipid content of dry bacterial cells were（53.67 ± 0.17） % and（13.55 ± 0.10） g/L,respectively,and the yield of lipid（7.27 ± 0.03） g/L fermentation broth.

关 键 词：红酵母 微生物油脂 鉴定 诱变 培养条件优化 

分 类 号：Q819[生物学—生物工程]