丛梗孢酵母产赤藓糖醇的诱变选育  

Mutation Breeding of an Erythritol-producing Moniliella acetoabutans Strain

作　　者：刘鹏[1] 王泽南[1] 李莹[1] 张秋子[1] 吴红引[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《食品科学》2011年第11期216-221,共6页Food Science

基　　金：安徽高校省级自然科学研究重点项目(Kj2010A276)

摘　　要：利用微波-硫酸二乙酯复合诱变对产赤藓糖醇丛梗孢酵母E54进行处理,以高渗平板和摇瓶发酵为筛选方法,得到遗传稳定的诱变高产株EW29;再采用氮离子注入对EW29进行诱变处理,摇瓶发酵筛选得到诱变株EN59,其90h发酵液中赤藓糖醇产量达到55.13g/L,较EW29提高20.3%,较E54提高36.9%,遗传稳定性较好。对突变株EN59的发酵培养基进行了优化,在优化培养基葡萄糖250g/L、酵母膏5g/L、KH2PO4 0.3g/L、MnSO4.4H2O0.04g/L、CuSO4.5H2O 0.03g/L,初始pH4的条件下,90h发酵液中赤藓糖醇平均产量达到69.00g/L以上。在优化培养基的基础上进行5L罐发酵放大实验,发酵126h赤藓糖醇产量达到71.14g/L。A genetically stable strain,named EW29,was obtained using Moniliella acetoabutans E54 as the starting strain by microwave-DES mutagenesis followed by screening by high osmotic plate culture and shake flask culture.The stain was further mutagenized by nitrogen ion implantation and then a strain EN59 was screened out of the resulting mutants by shake flask culture.The strain EN59 could produce 55.13 g/L erythritol after 90 h of fermentation,which was increased by 20.3% compared with EW29 and 36.9% compared with E54.The fermentation medium composition for EN59 was optimized to be 250 g/L glucose,5 g/L yeast extract,0.3 g/L KH2PO4,0.04 g/L MnSO4·4H2O,0.03 g/L CuSO4·5H2O and pH 4.The concentration of erythritol was over 69.00 g/L after 90 h fermentation under the optimal condition.The final concentration of erythritol reached up to 71.14 g/L after 126 h fermentation in 5 L fermentor.

关 键 词：复合诱变 氮离子注入 赤藓糖醇 丛梗孢酵母 

分 类 号：Q939.97[生物学—微生物学]