大口径毛细管气相色谱法快速测定MRS肉汤培养基中水溶性胆固醇  

Rapid Determination of Water-Soluble Cholesterol in MRS Broth by Wide-Bore Capillary Gas Chromatography

作　　者：郭春锋[1] 张兰威[1] 李婧妍[1] 张英春[1] 易华西[1] 杜明[1] 韩雪[1] 

机构地区：[1]哈尔滨工业大学食品科学与工程学院,哈尔滨150090

出　　处：《分析化学》2011年第6期894-897,共4页Chinese Journal of Analytical Chemistry

基　　金：国家高技术研究发展计划(No.2007AAZ354);国家科技支撑计划"十一五"奶业重大专项(No.2006BAD04A09)资助

摘　　要：建立了MRS肉汤培养基中水溶性胆固醇的气相色谱测定法。1 mL样品(含水溶性胆固醇的MRS肉汤培养基)与5 mL 0.5 mol/L KOH-甲醇溶液混合,然后于60℃水浴皂化30 min。产物游离胆固醇经5 mL甲苯萃取后,采用HP-5大口径石英毛细管柱(30 m×0.53 mm i.d.×1.5μm)进行分离、FID检测器进行检测、外标法进行定量分析。本方法分析一个样品仅需45 min;在5～250 mg/L浓度范围内线性关系良好;回收率为95.6%～98.0%;日内与日间相对标准偏差分别为1.6%和1.9%。本方法具有快速、简便、准确等优点。A method for water-soluble cholesterol quantification in de Man-Rogosa-Sharpe（MRS） broth based on capillary gas chromatography was developed.Samples（1 mL） were saponified in capped tubes with 5 mL methanolic KOH solution（0.5 mol/L） by heating for 30 min at 60 ℃.The product（cholesterol） was extracted with toluene,and directly analyzed by gas chromatography.The analysis was carried out using a wide-bore column HP-5（30 m × 0.53 mm i.d.× 1.5 μm）,FID detector and external standard method for quantification.With this method,a single sample could be analyzed in 45 min.The linearity was excellent for the concentration range of 5-250 mg/L.The recovery and overall relative standard deviation（RSD） were 95.6% 98.0% and 1.6%,respectively.This method is rapid,simple and accurate.

关 键 词：大口径毛细管 气相色谱 水溶性胆固醇 MRS肉汤培养基 

分 类 号：TS207.3[轻工技术与工程—食品科学]