来源于瓶霉Phialophora sp. G5的酸性木聚糖酶基因的克隆及性质的研究  

Studies on Cloning of Acid Xylanase from Phialophora sp. G5 and its Characteristics

作　　者：张帆[1,2] 石鹏君[2] 缪礼鸿[1] 姚斌[2] 

机构地区：[1]武汉工业学院生物与制药工程学院,武汉430023 [2]中国农业科学院饲料研究所,农业部饲料生物技术重点实验室,北京100081

出　　处：《中国农业科技导报》2011年第3期60-66,共7页Journal of Agricultural Science and Technology

基　　金：国家转基因生物新品种培育重大专项(2009ZX08003-020B);国家肉鸡产业技术体系(nycytx-42-G2-05)资助

摘　　要：通过简并PCR和TAIL-PCR技术从瓶霉Phialophora sp. G5菌株基因组DNA中克隆得到一个新的编码b-1,4-木聚糖酶的基因,命名为xyn11G5。该基因ORF全长879 bp,共编码292个氨基酸和一个终止密码子,前19个氨基酸为信号肽序列。将xyn11G5在毕赤酵母中进行分泌表达,重组蛋白经纯化达到电泳纯。酶学性质分析表明,该重组木聚糖酶最适温度为50℃,最适pH为5.0,在中性条件下具有良好的稳定性,并且具有一定的热稳定性,在饲料行业和生物能源行业领域具有潜在的应用前景。A xylanase gene xyn11G5 was cloned from Phialophora sp.G5 by degenerate PCR and TAIL-PCR method.Its ORF was 879 bp,encoded 292 amino acids residues,and the frontal 19 residues composed a putative signal peptide.Xyn11G5 was successfully expressed in Pichia pastoris and purified.The enzymatic characteristics analysis showed that the optimum pH and temperature for the recombinant xylanase was pH 5.0 and 50℃,respectively.It showed pH stability under neutral and thermo stability.Xyn11G5 was a good candidate in animal feed and bio-fuel industrial application.

关 键 词：PHIALOPHORA sp. G5 木聚糖酶 毕赤酵母 酶学性质 

分 类 号：Q78[生物学—分子生物学]