基于SNPs标记的猪肉DNA溯源技术的研究  

Studies on DNA Technology for Pork Traceability Based on SNPs Markers

作　　者：张小波[1,2] 吴潇[2,3] 何慧[1] 朱连龙[2,3] 唐雪明[2,3] 

机构地区：[1]华中农业大学食品科技学院,武汉430070 [2]上海市农业科学院生物技术研究所,上海201106 [3]上海市农业遗传育种重点实验室,上海201106

出　　处：《中国农业科技导报》2011年第3期85-91,共7页Journal of Agricultural Science and Technology

基　　金：国家863计划项目(2008AA100804);上海市自然科学基金项目(09ZR1428000);上海市启明星计划项目(10QA1406000);上海市科委创新平台建设项目(10DZ2294103)资助

摘　　要：建立溯源系统对肉类食品的管理和质量安全控制具有十分重要的作用。传统标记方法在肉类溯源的应用中受到了限制,基于生物个体本身基因组DNA特性而建立的DNA溯源标记方法可以解决这一难题。在国内首次对猪肉进行了基于SNPs标记的DNA溯源技术研究。采用RFLP-PCR方法检测了猪12个SNP候选标记,以期寻找到多态性信息含量丰富的SNP位点用于猪肉DNA溯源标记,结果发现6个SNP位点符合预期标准,分别是SNP1,SNP2,SNP3,SNP4,SNP5和SNP12,为建立猪肉产品的DNA溯源系统奠定了基础。It is very important to establish a traceability system for meat quality management and safety control.The traditional marker methods have limitations in application of meat traceability,and the new DNA genetic marker method based on characteristics of genomic DNA itself could overcome this limitation.This is the first study investigating the DNA traceability for pork based on SNPs markers in China.We have tested 12 candidate SNP loci by RFLP-PCR to look after the SNP with abundant polymorphic information for the DNA traceability.The results showed that 6 SNP loci meet the expected standard,namely SNP1,SNP2,SNP3,SNP4,SNP5 and SNP12,which provided a basis for the establishment of traceability marker system in Shanghai.

关 键 词：猪肉 食品安全 DNA溯源 SNP 

分 类 号：S828[农业科学—畜牧学] TS207.7[农业科学—畜牧兽医]