甜高粱秸秆发酵菌种的诱变育种及其固态发酵工艺的研究  

Study on Mutation Breeding of Fermenting Strains for Sweet Sorghum Stalk & Its Solid Fermentation Techniques

作　　者：刘健[1,2] 叶凯 陈美珍[1] 陈高云[2] 高小燕[1] 涂振东 刘敏[2] 

机构地区：[1]汕头大学理学院生物系,广东汕头515063 [2]中国人民解放军防化指挥工程学院三系生物防护教研室,北京102205 [3]新疆维吾尔自治区农科院,新疆乌鲁木齐830091

出　　处：《酿酒科技》2011年第6期28-31,共4页Liquor-Making Science & Technology

基　　金：甜高粱秸秆五碳糖发酵菌株引进与基因工程改造(2010-Z46);现代高粱产业技术体系建设任务书(nycytx-12-03-01-01);能源甜高粱产量与含糖量协同提高关键技术研究(3-30);新疆维吾尔自治区自然科学基金项目(200821175)

摘　　要：以耐酒精高活性干酵母为原始出发菌株进行60Co-γ辐射诱变处理,经逐级筛选和300 g甜高粱秸秆固态发酵,确定H13为最佳诱变菌种;并对H13菌株的甜高粱秸秆固态发酵工艺进行研究,结果表明,粉碎后的甜高粱秸秆300 g,5‰的菌体接种量、68%的基质含水量、36℃条件下发酵60 h,乙醇产率达6.4 g/100 g鲜秸秆。Angel active dry yeast（Saccharomyces cerevisiae） was selected as original strains and mutated by radiation of 60Co-γ,then H13 strain was obtained through screening and solid fermention test（fermented with 300 g sweet sorghum stalk）.The optimum fermentation conditions of H13 strain were summed up as follows through experiments： 300g grinding sweet sorghum stalk,5 ‰ bacteria inoculation quantity,68 % water content of sustrate,60 h fermentation at 36 ℃,and ethanol yield could reach 6.4 g/100 g stalk.

关 键 词：微生物 甜高粱秸秆 60Co-γ辐射诱变 固态发酵 

分 类 号：TQ223.122[化学工程—有机化工]