石花菜多糖的提取工艺及其抗氧化活性研究  

Study on the Extraction and Antioxidant Activity of Polysaccharides from Gelidium amansii

作　　者：许瑞波[1,2] 吴琳[1] 王吉[1] 周洪英[1] 巩建龙[1] 史大华[1] 

机构地区：[1]淮海工学院化学工程学院,江苏连云港222005 [2]淮海工学院江苏省海洋生物技术重点建设实验室,江苏连云港222005

出　　处：《淮海工学院学报（自然科学版）》2011年第2期38-41,共4页Journal of Huaihai Institute of Technology:Natural Sciences Edition

基　　金：江苏省自然科学基金(企业博士创新)项目(BK2009640);江苏省海洋生物技术重点建设实验室开放课题(2009HS06);江苏省青蓝工程优秀青年骨干教师资助项目(2008);淮海工学院博士科研启动项目(KQ10130)

摘　　要：利用水提醇沉法提取石花菜多糖(GAP),通过单因素和正交实验考察了温度、时间、料液比和提取次数等因素对GAP提取率的影响。结果表明,GAP的最适宜提取工艺条件是:料液比为1g∶30mL,温度75℃,时间2h,提取3次。GAP对羟基自由基、超氧阴离子自由基的清除实验表明,GAP具有良好的抗氧化活性,而且对2种自由基的清除能力强弱顺序是.OH>O2-.。Polysaccharides（GAP） was extracted from Gelidium amansii by water-extraction and ethanol precipitation method.The effects of extraction temperature,time,the ratio of raw material to water and extraction times on the yield of GAP were investigated by single factor and orthogonal experiments.The results showed that the optimum extraction conditions were as following： the ratio of raw material to water is 1g∶30mL,extraction temperature 75℃,extraction time 2h,and frequency of extraction 3 times.The scavenging capacity of GAP to hydroxyl free radical and superoxide anion free radical were studied,and the results indicated that GAP had good antioxidant activity and its scavenging capacity to ·OH was much higher than that of O2-·.

关 键 词：石花菜 多糖 提取 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]