微生物发酵法生产2,3-丁二醇的研究  

Production of 2,3-Butanediol by Microorganism Fermentation

作　　者：蒋丽群[1,2] 郭峰[1] 方真[1] 龙运多[1] 张帆[1] 

机构地区：[1]中国科学院西双版纳热带植物园热带植物资源开放实验室生物能源组,云南昆明650223 [2]中国科学院研究生院,北京100049

出　　处：《化学与生物工程》2011年第6期25-28,共4页Chemistry & Bioengineering

基　　金：国家自然科学基金资助项目(21076220);中国科学院知识创新工程重要方向性项目(KSCX2-YW-G-075)

摘　　要：采用响应面法(RSM)对产酸克雷伯氏菌发酵葡萄糖生产2,3-丁二醇的培养基进行了优化。依次通过Plackett-Burman实验、最陡爬坡实验和中心复合实验确定尿素和乙酸的最佳组合为:尿素浓度5.13 g.L-1、乙酸浓度0.703 g.L-1。在最佳发酵条件下,2,3-丁二醇得率达0.456 g.g-1,较初始条件提高了23.2%,达到了理论得率的91.2%。对进一步降低2,3-丁二醇的生产成本具有一定的指导意义,为木质纤维素资源的开发和利用奠定了良好的基础。Production of 2,3-butanediol from glucose by Klebsiella oxytoca fermentation was optimized using response surface methodology（RSM）.Based on the Plackett-Burman experiment,urea and acetic acid were identified as the most significant factors.Steepest ascent experiment and central composite experiment were employed to determine their optimal levels with urea concentration of 5.13 g·L-1 and acetic acid concentration of 0.703 g·L-1.Under the optimal medium,2,3-butanediol yield was 0.456 g·g-1,which was equivalent to 91.2% of the theoretical value and was 23.2% higher than that obtained under the initial conditions.In this work,a relatively high yield was achieved by using urea as sole nitrogen source that was less expensive,and the process may find practical applications for 2,3-butanediol production.

关 键 词：2 3-丁二醇 葡萄糖 响应面法 微生物发酵 

分 类 号：TQ923[轻工技术与工程—发酵工程]