竹质纤维素的酶解糖化条件研究  

Enzymatic saccharification conditions of bamboo cellulose

作　　者：李文华[1] 温忠文[1] 涂国全[1] 

机构地区：[1]江西农业大学生物科学与工程学院,江西南昌330045

出　　处：《中国酿造》2011年第6期51-53,共3页China Brewing

基　　金：江西省科技支撑计划项目(0905192378)

摘　　要：为探明竹质纤维素的酶解糖化较优工艺条件,先将竹粉与2.0%稀硫酸比例1∶15,80℃水浴20h成竹粉稀酸水解液,再用烧碱分别调节不同起始pH值,进行不同酶解温度、纤维素酶与木聚糖酶配比、摇床转速和酶解时间的单因素酶解糖化试验,测定并计算过滤清液中还原糖和总糖含量与得率。结果表明:竹粉酸解液中溶出还原糖和总糖得率比室温时分别提高5.20倍和6.43倍。初步探明竹粉酸解液的酶解条件:竹粉酸解液起始pH值5.5,纤维素酶与木聚糖酶配比为1∶1,摇床转速100r/min,温度50℃,时间24h,酶解后还原糖和总糖得率分别提高4.4倍和3.0倍,均超过50.88%。The optimal conditions for enzymatic saccharification of bamboo cellulose were studied. Prior to enzymatic hydrolysis, the acid hydrolysate of bamboo powder was prepared by adding bamboo powder in 2.0% H2SO4 at the ratio of 1：15 for a water bath at 80℃ for 20 h. Then, the initial pH of the acid hydrolysate for enzymatic saccharification was regulated by NaOH. Single factor tests for the optimization of enzymatic saccharification of bamboo cellulose were performed on different treating conditions such as initial pH, enzymatic hydrolysis temperature, cellulase-xylanase ratio, rotational speed and hydrolysis time. The reducing sugar and total sugar contents and yields in filtrate were detected. The results showed that the yields of reducing sugar and total sugar in the acid hydrolysate treated at 80℃ were 5.20 times and 6.43 times higher than those of the hydrolysated prepared under room temperature, respectively. The enzymatic saccharification conditions of the acid hydrolysate of bamboo powder were as follows： initial pH 5.5, cellulase-xylanase ratio 1：1, rotational speed 100 r/min, enzymatic hydrolysis temperature 50℃ and enzymatic hydrolysis time 24h. The yields of reducing sugar and total sugar under the optimal conditions were improved by 4.4 times and 3.0 times after enzymatic hydrolysis respectively, and both were higher than 50.88%.

关 键 词：竹质纤维素 预处理 纤维素酶 木聚糖酶 酶解糖化 

分 类 号：O556[理学—热学与物质分子运动论]