反应介质对脂肪酶活力及催化猪油水解和甲醇解的影响  

Effect of reaction media on lipase activity and lipase-catalyzed hydrolysis and methanolysis of lard

作　　者：张瑶瑶[1] 孙玉梅[1] 曹方[1] 杜巧娟[1] 张晓锋[1] 陈敏[2] 

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034 [2]大连工业大学化工与材料学院,辽宁大连116034

出　　处：《中国酿造》2011年第6期103-106,共4页China Brewing

基　　金：辽宁省教育厅2006年创新团队项目(2006T030)

摘　　要：研究了反应介质对脂肪酶活力及其催化猪油水解和甲醇解的影响,反应介质包括有机溶剂、缓冲液和甘油。实验结果表明,在脂肪酶催化猪油水解和甲醇解体系中,反应介质对脂肪酶活力和猪油转化率影响很大,添加20%(v/v)的正己烷比不添加正己烷的转化率提高8.8%,在40%的正己烷体系中添加20%(v/v)50mmol/L、pH 6.8的磷酸缓冲液比添加等量水的转化率提高29.2%,在40%的正己烷体系和20%的磷酸缓冲液体系中添加1.83%(v/v)的甘油比不添加甘油的转化率提高17.2%。The effect of reaction media on lipase activity and lipase-catalyzed hydrolysis and methanolysis of lard was studied. The reaction media included organic solvents, buffers and glycerol. The results indicated that the reaction media had significant effect on lipase activity and conversion rate of lard both in hydrolysis and methanolysis. When 20% （v/v）n-hexane was added, the conversion rate was 8.8% higher than that without n-hexane added. In the reaction system containing 40% n-hexane, the conversion rate increased by 29.2% when 20% （v/v） phosphate buffer （50mmol ·L^-1, pH 6.8）was added compared to the addition of equal volume of water. Furthermore, the conversion rate increased by 17.2% when 1.83% （v/v）glycerol was added in the reaction system containing 40% n-hexane and 20% phosphate buffer .

关 键 词：反应介质 脂肪酶 猪油 水解 甲醇解 

分 类 号：Q556[生物学—生物化学]