高速逆流色谱法分离花椒生物碱  

Separation of alkaloids from Zanthoxylum bungeanum Maxim.by high-speed countercurrent chromatography

作　　者：关荣琴[1] 张鸣镝[1] 石雪萍[2] 张卫明[2] 

机构地区：[1]吉林大学军需科技学院,吉林长春130062 [2]南京野生植物综合利用研究院,江苏南京210042

出　　处：《食品工业科技》2011年第7期257-259,共3页Science and Technology of Food Industry

基　　金：国家"十一五"科技支撑计划课题(2006BAD06B02)

摘　　要：采用高速逆流色谱法分离花椒中的生物碱。逆流色谱条件:正己烷-乙酸乙酯-甲醇-水(5∶5∶7∶5)为溶剂体系,上相为固定相,下相为流动相,流速2mL/min,仪器转速800r/min,检测波长254nm,上样量800mg。在此条件下,一次分离得到组分A(130mg)和组分B(15mg),经薄层色谱定性分析为生物碱,HPLC测定组分A和B的纯度分别为92.8%、85.4%。High- speed counter- current chromatography （HSCCC） with a two- phase solvent system composed of n- hexane- ethyl acetate- methanol- water （ 5：5 ：7 ：7, v/v） was applied to the isolation and purification of alkaloids from Zanthoxylum bungeanum Maxim.The lower phase was used as the mobile phase and performed at a flow rate of 2mL/min,while the apparatus rotated at 800r/min, and detected at 254nm.Two kinds of alkaloids were obtained and yielded 130mg（A）,15mg（B）from 800mg of crude extract in a one-step separation.A and B were identified by Thin Layer chromatography（TLC） ,and the results showed that the two fractions were alkaloids,with the purity of 92.8% and 85.4% ,determined by high performance liquid chromatography（HPLC）.

关 键 词：花椒 生物碱 高速逆流色谱 高效液相色谱 

分 类 号：TS201.1[轻工技术与工程—食品科学]