蓝色犁头霉深层发酵产壳低聚糖生长动力学研究  

Kinetic Study of the Low-molecular-chitosan Formation by Absidia coerulea

作　　者：王伟平[1] 陈维[1] 韩凤云[1] 杜予民[2] 

机构地区：[1]湖北工业大学生物工程学院,湖北武汉430068 [2]武汉大学资源与环境科学学院,湖北武汉430072

出　　处：《生物技术》2011年第3期82-84,共3页Biotechnology

摘　　要：目的:研究蓝色犁头霉产壳低聚糖分批发酵动力学的模型。方法:在10L发酵罐中,将蓝色犁头霉分批发酵培养,对蓝色犁头霉菌丝体生长、产物形成和基质消耗的实验数据进行分析,根据Logistic和Luedeking-Piret方程分别建立蓝色犁头霉发酵过程菌体生长、壳低聚糖生成和基质消耗的动力学数学模型,并利用1stOpt软件对模型参数进行非线性曲线拟合。结果:研究得到了菌体生长、产物合成及基质消耗的动力学模型及参数,模型的拟合度分别为0.994、0.986、0.992。结论:研究表明蓝色犁头霉多糖合成和菌体生长呈生长偶联型,模型计算值与实验值有良好的拟合性,模型准确度较高。Objective：To evaluate the kinetic models of Absidia coerulea producing low molecular chitosan （LMC） in Batch fermentation. Method：Absidia coendea was cultured in 10L fermentor, and then the data of cell growth, product formation and substrate consumption were analyzed. Based on the Logistic and Luedeking - Piret equations, the mathematic models of cell growth, LMC production and substrate consumption were developed to describe the Absidia coerulea fermentation process. The parameters of the models were fitted by lstOpt software using non -linear fitting techniques. Result： The mathematic models and the parameters of cell growth, LMC production and substrate consumption were made and the fitting degree was 0.994, 0.986 and 0.992 respectively. Conclusion： The results showed the production of LMC was growth - associated and the process of Absidia coerulea fermentation was well reflected by the mathematic models of cell growth, LMC production and substrate consumption.

关 键 词：蓝色犁头霉 壳低聚糖 1stOpt 发酵动力学模型 

分 类 号：TQ929.2[轻工技术与工程—发酵工程]