一株甲基对硫磷降解菌——米曲霉JMUPMD-2的分离与鉴定  

Isolation and identification of a parathion-methyl degrading fungus-Aspergillus oryzae JMUPMD-2

作　　者：解顺昌[1] 倪辉[1] 蔡薇[1] 曹樱[1] 李利君[1] 肖安风[1] 黄高凌[1] 杜凤君 蔡慧农[1] 

机构地区：[1]集美大学生物工程学院,福建厦门361021 [2]厦门市出入境检验检疫局,福建厦门361026

出　　处：《微生物学通报》2011年第7期1007-1013,共7页Microbiology China

基　　金：集美大学中青年创新团队专项基金(No.2008A002)

摘　　要：对一株新分离的、能以甲基对硫磷为唯一磷源生长的菌株——JMUPMD-2利用形态观察和rDNA ITS序列分析对JMUPMD-2进行鉴定;用气相色谱法检测培养过程中甲基对硫磷浓度的变化,确定甲基对硫磷降解速率。该菌株的rDNA ITS序列与米曲霉(Aspergillus oryzae)的同源性为99%,菌落形态和显微形态都与米曲霉特征相符,因此鉴定为米曲霉;以350μg/L甲基对硫磷为唯一磷源和350μg/L甲基对硫磷及1 g/L K2HPO4组成的混合磷源分别培养该菌株,200 h后的分解量分别为189μg/L及28μg/L。该菌株的胞内提取液具有明显的甲基对硫磷降解酶活性。A newly isolated fungus JMUPMD-2,capable of growing with parathion-methyl as the sole phosphorus source,was identified based on morphological charaterisitics and rDNA ITS sequences analysis,and its capability of degrading parathion-methyl was investigated by determining the changes of parathion-methyl concentration in the broth of the isolate.JMUPMD-2＇s colonic and microscopic morphologies were identical with and its sequences of rDNA ITS was 99% similar to Apspergillus oryzae.Consequently,it was identified as Aspergillus oryzae.When cultured with an artificial medium containing parathion-methyl as the sole phosphorus or parathion-methyl-K2HPO4 mixed phosphorus,concentration of parathion-methyl respectively reduced 189 μg/L and 28 μg/L after inoculating for 200 hours.JMUPMD-2＇s intracellular extract had obvious parathion-methyl degrading enzyme activity.

关 键 词：甲基对硫磷 生物降解 ITS序列 米曲霉 菌种鉴定 

分 类 号：X172[环境科学与工程—环境科学]