HPLC-UV法测定微生物降解体系中3-苯氧基苯甲酸含量  

Determination of 3-Phenoxybenzoic Acid in Microbial Degradation Systems by HPLC-UV Detection

作　　者：赵楠[1] 刘书亮[1] 赖文[1] 袁怀瑜[1] 

机构地区：[1]四川农业大学食品学院,四川雅安625014

出　　处：《食品科学》2011年第14期181-184,共4页Food Science

基　　金：国家自然科学基金面上项目(21072137)

摘　　要：采用高效液相色谱-紫外(high-performance liquid chromatography with ultraviolet,HPLC-UV)法测定微生物降解体系中3-苯氧基苯甲酸(3-PBA)的含量。以Gemini 100A C18柱(150mm×4.60mm,5.0μm)为色谱柱,乙腈-水(70:30,V/V)为流动相,流速0.7mL/min,用紫外检测器在210nm处检测3-PBA。结果显示:3-PBA对照品的保留时间为4.268min,线性范围为0.5～50.0mg/L,3-PBA平均回收率为98.919%,RSD为2.78%;运用该方法测得4种不同来源的混合菌株发酵液中3-PBA的残留量分别为24.467、86.266、2.633、1.921mg/L。本法简单、快速、准确、分离度好。A HPLC method with UV detection was proposed to determine the concentration of 3-phenoxybenzoic acid （3-PBA） in microbial degradation systems. The chromatographic separation was performed on a Gemini 100A C18 column （5.0 μm, 150 mm× 4.60 mm, i.d.） using a mobile phase made up of acetonitrile and water （70：30, V/V） at a flow rate of 0.7 mL/min, and 3- PBA was detected at 210 nm using a UV detector. The retention time of 3-PBA was 4.268 min. The linear range of the method was 0.5--50.0 mg/L. The average spike recovery for 3-PBA was 98.919%, with a relative standard deviation （RSD） of 2.78%. The residues of 3-PBA in 4 different microbial degradation systems were 24.467, 86.266, 2.633 mg/L and 1.921 mg/L, respectively. The developed method demonstrated the benefits of simplicity, rapidity, high accuracy and good separation.

关 键 词：高效液相色谱-紫外(HPLC—UV法) 3-苯氧基苯甲酸(3-PBA) 微生物降解体系 检测 

分 类 号：TS201.3[轻工技术与工程—食品科学]