一株产纤维素酶真菌的筛选、鉴定及降解效果  

Screening and Identification of a Prolific Fungus Strain Producing Cellulase and Its Degradation Effect

作　　者：李保深[1] 高大文[1] 张博[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《东北林业大学学报》2011年第7期134-137,共4页Journal of Northeast Forestry University

基　　金：全国优秀博士学位论文专项基金(200544);黑龙江省杰出青年基金(JC200909)

摘　　要：从东北地区自然环境中采集不同区域腐殖质样品,筛选分离得到1株产纤维素酶的真菌C5,经形态学、生理生化特征试验,结合18S rDNA基因序列分析及扫描电镜分析鉴定该菌为斜卧青霉(Penicillium decum-bens),对其产酶能力及其对秸秆降解效果的研究结果表明:真菌C5具有较全的纤维素酶系和较高的纤维素酶活力,对秸秆纤维素具有很好的降解效果。该菌株在以玉米秸秆为唯一碳源,28℃、120 r/min的条件下发酵培养至第3天时,滤纸酶活最高为7.085 IU/mL,内切葡聚糖酶、外切葡聚糖酶及β-葡萄糖苷酶最高值分别为3.856、2.681、1.487 IU/mL。Humus samples were collected from different regions in the natural environment of northeast China. A cellulase-producing fungus C5 was obtained through screening and isolation. The fungus was identified as Penicillium decumbens according to its morphological, biochemical and physiological characteristics, combined with the analyses of 18S rDNA gene sequences and scanning electron microscope. Moreover, the abilities to produce enzyme and degrade straw for the strain C5 were studied. Results show that C5 has a relatively complete cellulase system and a high cellulase activity. C5 could degrade straw cellulose efficiently. When corn straw was served as the sole carbon source for the growing of the strain, the filter paper lyase （FPase） activity could achieve its peak value （7. 085 IU/mL） after three days of fermentation under the conditions of 120 r/min at 28 degrees C. The peak Values of endoglucanase （ EG/Cen）, exoglucanase （CBH/Cex） and β- glycosidase （β-Gase） were 3. 856 , 2. 681 and 1. 487 IU/mL respectively.

关 键 词：筛选分离 斜卧青霉 18S RDNA 纤维素酶 扫描电镜 

分 类 号：Q939.96[生物学—微生物学]