SephadexG-25对羊骨胶原降血压肽的分离纯化效果研究  

Sephadexg-25 Column Chromatographic Separation,MALDI-TOF-MS Analysis and Amino Acid Composition of Sheep Bone Collagen-derived ACE Inhibitory Peptides

作　　者：冯雅蓉[1] 马俪珍[2] 李黎[3] 

机构地区：[1]山西农业大学食品科学与工程学院,山西太谷030801 [2]天津农学院食品科学系,天津300384 [3]天津天狮学院生物工程学院,天津301700

出　　处：《肉类研究》2011年第6期30-33,共4页Meat Research

基　　金：天津市科技攻关项目(05ZHGCNC00100)

摘　　要：利用中性蛋白酶酶解羊骨得到羊骨胶原降血压肽粗品的基础上,研究Sephadex G-25凝胶过滤层析方法对羊骨胶原降血压肽粗品的分离纯化效果。结果显示:Sephadex G-25凝胶过滤层析将分子质量大于5000D的大分子滤去,得到了峰1和峰2两种多肽产品,其血管紧张素转化酶(ACE)抑制率均达到92%以上。采用飞行质谱对峰1和峰2多肽产品的分子质量进行质谱测定,肽质量指纹图谱显示,利用SephadexG-25凝胶过滤层析方法,并不能得到羊骨胶原降血压肽纯品,峰1和峰2均由多组分组成,分别出现30多个片段,其中峰1中肽峰最高处的分子质量为1077.36D,而峰2中肽峰最高处的分子质量为1048.47D。峰1的氨基酸总量为23.49g/100g,而峰2的氨基酸总量为8.32g/100g,两个峰均含有人体所需要的8种必需氨基酸,且甘氨酸、脯氨酸和谷氨酸3种非必需氨基酸含量相对比较高。Sephadexg-25 column chromatography was used to separate hydrolyzed sheep bone collagen prepared with neutral protease to molecules of larger than 5000 D in size and two ACE inhibitory peptides, peaks 1 and 2, both of which had an average inhibitory rate of over 92%. MALDI-TOF-MS （matrix assisted laser desorption ionization/time of flight MS） analysis showed that both peaks consisted of more than 30 fragments and the peptide showing the highest intensity was 1077.36 D in peak 1 and 1048.47D in peak 2. The total amino acid content of peak 1 was 23.49 g/100 g and of peak 28.32 g/100 g. Eight essential amino acids were found in both of them, and three nonessential amino acids such as Gly, Pro and Ghi were also abundantly contained.

关 键 词：羊骨 降血压肽 凝胶过滤层析 飞行质谱 

分 类 号：TQ464.7[化学工程—制药化工]