反相高效液相色谱法测定油料饼粕氨基酸含量  

Measurement of amino acids in oilseed meals by reversed-phase high performance liquid chromatography

作　　者：栾霞[1] 初雷[1,2] 李爱科[1] 王瑛瑶[1] 

机构地区：[1]国家粮食局科学研究院,北京100037 [2]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《粮油食品科技》2011年第3期30-32,共3页Science and Technology of Cereals,Oils and Foods

基　　金：国家科技支撑计划-农副产品饲料转化与高效利用关键技术研究(2006BAD12B04)

摘　　要：采用反相高效液相色谱(RP-HPLC)法测定油料饼粕氨基酸含量,条件为:C_(18)(4.6mm×250mm,5μm)柱,流动相A为0.1mol/L的醋酸钠(pH6.5)-乙腈溶液(V/V185:14),流动相B为80%乙腈水溶液(V/V),流速为1mL/min,梯度洗脱,检测波长254 nm,柱温40℃。比较花生、菜籽和棉籽等饼粕样品中氨基酸组成和含量,分析结果表明菜籽饼粕氨基酸组成最合理,棉籽蛋白氨基酸含量最高。The amino acids in oilseed meal were determined by reversed-phase high performance liquid chromatography（RP-HPLC）.The samples were injected into reversed-phase column C_（18）（4.6 mm×250 mm,5μm）.The mobile phase A was 0.1mol/L sodium acetate（pH6.5）and acetonitrile（185/14, V/V）.The mobile phase B were water and acetonitrile（80%,V/V）.Gradient elution proceeded with flow rate of 1mL/min.The detection wavelength was 254 nm and the column temperature was maintained at 40℃.The peanut meal,rapeseed meal and cottonseed meal were determined with this method.The result showed that the amino acids ratio in rapeseed meal was most reasonable and the contents of amino acids in cottonseed meal were the highest.

关 键 词：HPLC法 饼粕 氨基酸 分析 

分 类 号：S816.43[农业科学—饲料科学]