啤酒生产废水培养斯达油脂酵母发酵产油脂培养基的优化  

Medium optimization of producing lipid with Lipomyces starkeyi from brewery wastewater

作　　者：王华[1,2] 刘欢[1,3] 郭书贤[4] 王冬梅[4] 薛刚[4] 

机构地区：[1]陕西省葡萄与葡萄酒工程技术研究中心,陕西杨凌712100 [2]西北农林科技大学葡萄酒学院,陕西杨凌712100 [3]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [4]南阳理工学院生物与化学工程学院,河南南阳473004

出　　处：《中国油脂》2011年第8期31-36,共6页China Oils and Fats

基　　金：河南省重点科技攻关项目(102102310033)

摘　　要：以啤酒生产废水及添加一定的营养因子为发酵基质,采用单因素和正交试验设计对斯达油脂酵母(Lipomyces starkeyi1809)发酵产油脂和COD降解的培养基进行了优化,并利用气相色谱分析比较了菌体油脂的脂肪酸组成。结果表明:最佳培养基组成为在啤酒生产废水中添加碳源和氮源的C/N为55∶1(即木糖28.88 g/L和硫酸铵1.0 g/L),FeC l3.6H2O 3 mg/L,色醇150μmol/L(在发酵36 h时添加),初始pH 5.5;用此培养基在摇床转速120 r/m in、温度28℃下培养斯达油脂酵母120 h,菌体生物量、油脂产量、油脂含量和废水的COD降解率分别达到12.28 g/L、4.40 g/L、35.80%和50.32%,生物量和油脂产量比优化前分别提高了137.98%和117.82%,所产菌体油脂的不饱和脂肪酸指数(IUFA)达到63.95%,其中油酸的相对含量高达56.29%,比优化前提高了5.45%,优化效果显著。Brewery wastewater and certain nutritional factors used as fermentation substrate were used to cultivate Lipomyces starkeyi 1809, the optimum medium for lipid production and degradation of COD was studied by single - factor and orthogonal experiments, and the fatty acids composition of microbial lipid was analyzed by GC. The results indicated that the best medium was composed of C/N 55：1 （xylose 28. 88 g/L, （ NH4 ）2SO4 1.0 g/L）, FeCl3 · 6H2O 3 mg/L, initial pH 5.5, tryptophl 150 μmol/L （ adding at 36 h）. After fermentation for 120 h at 28~C, 120 r/min and these conditions, the biomass was up to 12. 28 g/L, the lipid production was up to 4. 40 g/L, the lipid content was 35. 80%, and the degradation rate of COD was up to 50. 32%. The biomass and lipid production increased by 137. 98% and 117.82%, respectively. The IUFA of microbial lipid reached 63.95%, and the content of oleic acid was 56.29%,increasing by 5.45% than before. The results of optimization were obvious.

关 键 词：啤酒生产废水 斯达油脂酵母 微生物油脂 培养基优化 油脂产量 COD 

分 类 号：X792[环境科学与工程—环境工程]