聚合酶链反应-变性梯度凝胶电泳技术在口腔微生物多样性研究中的应用  

The application of PCR-DGGE on the research of oral cavity microbial diversity

作　　者：沈会平[1] 苏健裕[1] 彭帅[2] 朱啸[3] 石磊[1] 

机构地区：[1]华南理工大学轻工与食品学院,广州510640 [2]华南理工大学生物科学与工程学院,广州510640 [3]第四军医大学口腔医学院修复科,西安710032

出　　处：《检验医学与临床》2011年第16期1921-1922,1924,共3页Laboratory Medicine and Clinic

基　　金：广东省自然科学基金博士启动项目(10451064101005160)

摘　　要：目的应用聚合酶链反应(PCR)-变性梯度凝胶电泳(DGGE)技术,分析健康人的口腔菌群及其菌种组成的多样性。方法抽取8名健康人唾液样本,分别提取唾液细菌总DNA,PCR扩增16SrDNA并进行DGGE和系统发育树分析。结果 8名健康人唾液样本的DGGE指纹图谱条带差异性较大,仅有65%相似性,表明采用PCR-DGGE对口腔微生物多样性的检测是可行的。结论 PCR-DGGE技术可较灵敏、直观地反映口腔微生物多样性情况。Objective Polymerase chain reaction and denaturing gradient gel electrophoresis（PCR-DGGE） were applied to the microbial diversity examination of oral cavity.Methods Total DNAs were extracted from the plaques of 8 healthy people,16SrDNA of the bacteria was amplified and DGGE was also used to assess changes in bacterial composition from saliva microcosms from these 8 individual saliva samples.Results DGGE fingerprint patterns of saliva from 8 different donors displayed greater variability（65% concordance）.PCR-DGGE method was a viable approach to examine the microbial diversity of oral cavity.Conclusion These results suggest that PCR-DGGE method is a sensitive and effective approach to examine the microbial diversity of oral cavity.

关 键 词：微生物多样性 口腔微生态 变性梯度凝胶电泳 

分 类 号：R739.5[医药卫生—肿瘤]