乳酸提取鮰鱼皮胶原蛋白工艺优化  

Optimization of Conditions for Collagen Extraction with Lactic Acid from Channel Catfish(Ictalurus punctatus) Skin

作　　者：宫子慧[1] 林琳[1] 孟昌伟[1] 叶应旺[1] 姜绍通[1] 陆剑锋[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《肉类研究》2011年第7期18-23,共6页Meat Research

基　　金：安徽省"十一五"重大科技攻关专项(08010301078);国家星火计划项目(2008GA710021);安徽省自然科学基金项目(090411018);安徽高校省级自然科学研究重点项目(KJ2009A106);国家自然科学基金青年项目(31000832)

摘　　要：以斑点叉尾鮰鱼皮为实验材料,选用乳酸从中提取胶原蛋白。通过单因素试验分别考察pH值、料液比和时间3个参数对胶原蛋白提取率的影响,在此基础上,利用Box-Benhnken中心组合试验设计和响应面分析法对乳酸提取鮰鱼皮胶原蛋白条件进行优化。结果表明,乳酸提取鮰鱼皮胶原蛋白的优化工艺为pH2.3、料液比1:60(g/mL)、时间46h,在此条件下鮰鱼皮胶原蛋白的提取率达到46.28%。This study was undertaken to optimize the acidic extraction of collagen from channel catfish （lctalurus punctatus） skin. Lactic acid was found to be a better solvent for extracting collagen from channel catfish skin than formic acid, acetic acid, citric acid and hydrochloric acid. Further, three process conditions affecting the extraction rate of collagen with lactic acid, such as pH value, solid-to-liquid ratio and extraction time, were optimized by one-factor-at-a-time method and Box-Benhnken experimental design combined with response surface methodology. The results indicated that the optimal conditions for collagen extraction were pH 2.3, solid-to-liquid ratio 1：60 （g/mL） and extraction time 46 h, under which, an extraction rate of 46.28% was obtained.

关 键 词：鮰鱼皮 乳酸 胶原蛋白 响应面分析 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]