安琪酿酒酵母与管囊酵母原生质体制备和再生  

Preparation and Regeneration of Protoplast for Angel S.cerevisiae and Pachysolen tannophilus

作　　者：郑州[1] 田辉[1] 程金花[1] 赵儒铭[1] 李志军[2] 姚娟[2] 龚大春[1] 

机构地区：[1]三峡大学艾伦.麦克德尔米德再生能源研究所,湖北宜昌443002 [2]安琪酵母股份有限公司,湖北宜昌443002

出　　处：《酿酒科技》2011年第8期17-20,共4页Liquor-Making Science & Technology

基　　金：湖北省教育厅重点项目(CXY2009B008);国家自然基金项目(21076114)

摘　　要：通过紫外诱变筛选得到呼吸缺陷型酿酒酵母R40,再以R40和管囊酵母P01为亲本,进行原生质体制备与再生的研究,考察了酶浓度、酶解温度和酶解时间对两亲本原生质体制备和再生的影响。结果表明,当酶解时间为1 h,酶浓度为2%时,35℃下酶解酿酒酵母R40所得原生质体的形成率和再生率分别达到96.1%和12.5%;30℃下酶解管囊酵母P01所得原生质体的形成率和再生率为97.2%和10.1%。R40 S.cerevisiae of respiration defect type was screened out by UV mutagensis, and then the preparation and regeneration ofprotoplast with R40 S.cerevisiae and P01 Pachysolen tannophilus as parent strains were carried out and the effects of enzyme concentration, enzymatic hydrolysis temperature, and enzymatic hydrolysis time on the preparation and regeneration of protoplast were investigated. The results showed that as enzyme concentration was 2 % and enzymatic hydrolysis time was lh, the preparation rate and regeneration rate of protoplast for Angel R40 S. cerevisiae could reach up 96.1% and 12.5 % respectively at 35 ~C, and the preparation rate and regeneration rate of protoplast for P01 Pachysolen tannophilus could reach up 97.2 % and 10.1% respectively at 30 ℃.

关 键 词：微生物 安琪酿酒酵母 呼吸缺陷型 管囊酵母 原生质体 再生 

分 类 号：TS261.11[轻工技术与工程—发酵工程]