超高效液相色谱法同时测定油炸食品中4种黄曲霉毒素  

Simultaneous Detection of Aflatoxin B_1、B_2、G_1、G_2 in Fried Foods By Ultra Performance Liquid Chromatography

作　　者：张英[1] 蔡志斌[1] 郑志伟[1] 

机构地区：[1]深圳市龙岗区疾病预防控制中心,广东深圳518172

出　　处：《实用预防医学》2011年第8期1556-1559,共4页Practical Preventive Medicine

摘　　要：目的建立一种快速、灵敏、简便的超高效液相色谱法（UPLC）同时测定油炸食品中黄曲霉毒素B1（AFB1）、B2（AFB2）、G1（AFG1）、G2（AFG2）水平的方法。方法样品用乙腈水（84：16）提取液提取后过滤,滤液经黄曲霉毒素多功能净化柱（MFC）净化,纯化液经浓缩挥干后,加三氟乙酸（TFA）衍生后,用带有荧光检测器的超高效液相色谱仪测定。结果 4种黄曲霉毒素5 min完全分离,线性范围质量浓度分别为0.10~100.00μg/L（AFB1、AFG1）、0.05~50.00μg/L（AFB2、AFG2）,最低检出质量分数分别为0.04μg/kg（AFB1、AFG1）、0.02μg/kg（AFB2、AFG2）,油炸食品回收率为82.0%~101.2%,相对标准偏差〈5%,r≥0.999。结论该方法简单、快速、灵敏度高,适用于油炸食品中4种黄曲霉毒素水平的同时测定。Objective To establish a rapid,sensitive and simple method for the determination of aflatoxin B1、B2、G1、G2 in fried foods by ultra performance liquid chromatography（UPLC）.Methods Sample was extracted with 84% acetonitrile and cleaned-up with the MFC.The purified solution was concentrated and evaporated to dryness.Aflatoxins were derivatized with TFA and detected and quantitated by UPLC with fluorescence detection.Results The four afltoxins could be separated in 5 minutes.The linear range of the method is 0.10~100.00μg/L for aflatoxin B1,G1,and 0.05~50.00μg/L for aflatox B2,G2,and the lowest detection limit was 0.04μg/kg for aflatoxin B1、G1 and 0.02μg/kg for B2,G2.The method has been successfully applied to determination of fried foods.The recoveries of aflatoxins was in the range of 82.0%~101.2% and the relative standard deviation was 5%,The correlation coefficient was ≥0.999.Conclusion This method is simple,high sensitive,accurate and rapid for aflatoxin B1、B2、G1、G2 detection in fried foods.

关 键 词：黄曲霉毒素 超高效液相色谱法 油炸食品 

分 类 号：R155[医药卫生—营养与食品卫生学]