多棘海盘车体壁胶原蛋白肽的制备及其抗氧化活性  

Preparation and Antioxidative Activity Study on Collagen Peptides from Asterias amurensis

作　　者：耿浩[1] 刘云玲[1] 马超[1] 王唱[1] 宋春霞[1] 

机构地区：[1]山东大学威海分校海洋学院,山东威海264209

出　　处：《食品与发酵工业》2011年第6期42-46,共5页Food and Fermentation Industries

基　　金：山东大学威海分校学生科研立项资助项目(A10025)

摘　　要：以多棘海盘车体壁为材料提取胶原蛋白,制备多棘海盘车胶原蛋白肽(AACPs),并研究其体内、外抗氧化活性。方法:酶法提取胶原蛋白,选取木瓜蛋白酶、无花果蛋白酶等6种酶,在最适温度和pH下水解胶原蛋白,通过比较各种酶水解物体外清除自由基能力和对兔肝的过氧脂质化的抑制作用,确定多棘海盘车胶原蛋白的适宜水解酶,及酶解工艺,选取体外活性最高的样品进行小鼠体内抗氧化实验(ig,7d),建立小鼠急性乙醇肝氧化模型,测定肝组织中MDA含量及SOD、GSH-Px活力,考察胶原蛋白肽的体内抗氧化活性。结果:木瓜蛋白酶酶解物清除自由基活性最高,对脂质过氧化的抑制作用最明显。中、高剂量AACPs能够显著降低急性酒精肝氧化小鼠肝组织中的MDA含量(P<0.01),显著提高SOD(P<0.05)、GSH-Px活力(P<0.01)。结论:制备多棘海盘车胶原蛋白肽(AACPs)的最佳酶为木瓜蛋白酶,AACPs具有明显的体内外抗氧化作用。Extracted collagen from integument of Asterias amurensis.Prepared Asterias amurensis Collagen Peptides（AACPs） using different enzymes,and investigated antioxidative activity of the AACPs both in vivo and in vitro.Method： The collagen was hydrolyzed by six different kind of enzymes in their optimal conditions.The best enzyme was determined according to AACPs＇ ability of free radical scavenging and anti-lipid peroxidation effect on rabbit liver.Orthogonal experiment was used to determine the optimum hydrolyzed condition.An acute ethanol-induced oxidative stress mice model was established and was used to evaluated the antioxidant ability of AACPs with the sample that has the highest activity（7d,ig）.The activity of SOD,GSH-PX and content of MDA in liver were tested in this study.Result： AACP hydrolyzed with papain has the highest anti-oxidative activity in vitro.Compared with ethanol group,medium-dose and high-dose AACPs significantly reduced the content of MDA（P 0.01） and increase the activity of SOD（P 0.05） and GSH-PX（P 0.01） in liver of mice.Conclusion： Papain was chosen as the best enzyme for producing Asterias amurensis collagen peptides and the obtained AACPs have obvious anti-oxidative activity both in vitro and vivo.

关 键 词：多棘海盘车 胶原蛋白肽 抗氧化 

分 类 号：TQ936.2[化学工程]