3种不同虾类虾头中酶的筛选  

Enzyme screening from three different kinds of shrimp heads

作　　者：吴燕燕[1] 王萍[1,2] 李来好[1] 杨贤庆[1] 刁石强[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]上海海洋大学食品学院,上海201306

出　　处：《广东农业科学》2011年第14期116-119,共4页Guangdong Agricultural Sciences

基　　金：国家科技支撑计划项目(2008BAD94B02);广东省重点项目(2005B33201002)

摘　　要：以凡纳滨对虾、罗氏沼虾、斑节对虾的加工下脚料——虾头为原料,通过酶的提取、酶活测定及酶含量的分析,对这3种虾类虾头中的蛋白酶、碱性磷酸酶、酸性磷酸酶、乙酰胆碱酯酶、超氧化物歧化酶、溶菌酶、多酚氧化酶等酶类进行筛选和评价。结果表明:3种虾类的虾头中凡纳滨对虾虾头的蛋白酶酶活力为39 615.8(±3 108.4)U/gprot,约为其他2种虾的2倍;而罗氏沼虾虾头的碱性磷酸酶、超氧化物歧化酶具有较高活性,斑节对虾虾头的乙酰胆碱酯酶含量较高,在3种虾类的虾头中均未检测到溶菌酶。Seven economic enzymes（protease,alkaline phosphatase,acidic phosphatase,acetylcholin esterase,superoxide dismutase,lysozyme and polyphenoloxidase）were extracted and analyzed from the shrimp heads,which were regard as shrimp processing waste ofLitopenaeus vannamei,Macrobrachium rosenbergii,Penaeus monodn.Enzyme activity and enzyme content were used as parameters toevaluate enzymes.The results showed that the enzyme activity of protease from Litopenaeus vanname was 39 615.8（±3 108.4）U/gprot abouttwice the other two kinds.The enzyme activity and content of acidic phosphatase,superoxide dismutase from Macrobrachium rosenbergii,and acetylcholin esterase from Penaeus monodn were higher.Lysozyme was not detected in all three kinds.

关 键 词：虾头 酶 筛选 

分 类 号：Q814.1[生物学—生物工程]