大豆蛋白偶联壳聚糖微球介质提取纳豆激酶的研究  

Extraction of Nattokinase by Soybean Protein Cross-linked Chitosan Microsphere

作　　者：苏俊彩[1] 董超[2] 史延茂[2] 张聪莎[2] 王辉[3] 

机构地区：[1]河北医科大学第三医院,河北石家庄050051 [2]河北省科学院生物研究所,河北石家庄050081 [3]河北工业大学化工学院,天津300130

出　　处：《大豆科学》2011年第4期652-656,662,共6页Soybean Science

基　　金：河北省科学技术研究与发展计划资助项目(08276413D)

摘　　要：采用液体反相悬浮法制取壳聚糖微球,在微球表面偶联纳豆激酶的亲和配体—大豆蛋白,作为纳豆溶栓酶的亲和吸附介质。结果表明:壳聚糖微球本身对纳豆激酶没有特异吸附,偶联大豆蛋白的壳聚糖微球对发酵粗酶液中的纳豆激酶的吸附性能符合Langmuir方程,吸附平衡时间约为60 min,最大吸附量为3 926.56 U·g-1。纳豆激酶发酵液的上样量与柱体积比为1∶9时,纳豆激酶的收率可以达到52.3%,纯化倍数可以达到18.1倍。Chitosan microspheres were obtained by inverse phase suspension method.After being cross-linked with soybean protein on its surface,the chitosan microspheres became affinity adsorbent of Nattokinase.This study verified that there were no affinity binding between the chitosan microspheres and Nattokinase.The affinity adsorption was researched between the soybean protein cross-linked microspheres and Nattokinase.Results indicated that the adsorption isotherm was accorded with Langmuir,the time of adsorption balance was 60 min,the maximum of adsorption capacity was 3926.56 U·g-1.When the ratio of sample weight and column volume was 1∶9,the purification fold and recovery of Nattokinase activity reached 18.1 times and 52.3%,respectively.

关 键 词：纳豆激酶 壳聚糖微球 大豆蛋白 偶联 亲和层析 

分 类 号：S565.1[农业科学—作物学]