废次烟叶中茄尼醇的提取纯化工艺研究  

Studies on Extraction and Purification Technology of Solanesol from Discarded Tobacco

作　　者：朱松[1] 张海波[2] 王洪新[3] 陈尚卫[1] 戴军[1] 

机构地区：[1]江南大学食品科学与技术国家重点实验室,江苏无锡214036 [2]安琪酵母股份有限公司,湖北宜昌443003 [3]江南大学食品学院,江苏无锡214036

出　　处：《安徽农业科学》2011年第21期13080-13082,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]探讨废次烟叶中茄尼醇的提取纯化工艺。[方法]采用超声波辅助提取技术,结合柱色谱法对废次烟草中茄尼醇的提取、分离和纯化进行了研究。[结果]通过正交试验获得茄尼醇最佳提取条件为:超声功率120W,超声时间60min,料液比1:10,提取温度70℃。提取液经皂化、酸化后,用正己烷萃取得到纯度为21.5%的茄尼醇粗品;粗品经重结晶后得到纯度为77.6%的茄尼醇产品;进一步通过柱色谱分离,石油醚-乙酸乙酯(8/1,V/V)洗脱,收集相关流分,结晶得到纯度为99.2%的茄尼醇产品,整个工艺过程中茄尼醇产品的得率为0.512%。[结论]应用超声波辅助提取废次烟叶中的茄尼醇,具有萃取速度快、效率高的优点,适合工业化生产。[Objective]The aim was to study isolation and purification technology of solanesol from discarded tobacco.[Method]The extraction,isolation and purification technology of solanesol from discarded tobacco were studied by adopting ultrasonic-assisted extraction technique and column chromatography.[Result]The optimum extraction conditions of solanesol from discarded tobacco were obtained through orthogonal experiments as follows：the u1trasonic power was 120 W;the extraction time was 60 min;the proportion of raw materials and solution was 1：10;and the extraction temperature was 70 ℃.Then solansol was extracted from solansol extract by hexane with purity of 21.5% after saponification and acidification;after that the solansol with 77.6% purity was obtained by recrystallization;finally the crude solansol was purified on silica column chromatography using petroleun ether/ethyl acetate（8 /1,V/V） as mobile phase.The purity of solanesol was 99.2%,and the yield of solanesol was 0.512%.[Conclusion]Ultrasonic-assisted extracting solanesol from discarded tobacco has advantages of fast speed and high efficiency,so it is suitable for industrial production.

关 键 词：废次烟叶 茄尼醇 超声波辅助提取 纯化 

分 类 号：S572[农业科学—烟草工业]