反式脂肪酸Trans C18∶1诱导内皮细胞凋亡的研究  

Apoptosis of Human Umbilical Vein Endothelial Cells Induced by Trans C18∶1

作　　者：邱斌[1] 刘蓉[1] 邓泽元[1] 范亚苇[1] 李静[1] 胡蒋宁[1] 黎玉[2] 

机构地区：[1]食品科学与技术国家重点实验室,南昌大学,江西南昌330047 [2]南昌大学生命科学与食品工程学院,江西南昌330047

出　　处：《食品与生物技术学报》2011年第4期588-591,共4页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(30972482);江西省学术带头人计划项目(2008DD00900);教育部博士点基金项目(20070403002);江西省自然科学基金项目(2008GQY0023)

摘　　要：通过检测相关细胞凋亡指标,探讨trans C18:1诱导内皮细胞的凋亡机制。采用体外细胞培养方法将不同浓度trans C18∶1与内皮细胞共同孵育后,通过吉姆萨染色,观察内皮细胞形态变化;采用流式细胞仪检测细胞的早期凋亡变化;通过试剂盒检测观察trans C18:1对凋亡酶Caspase活力的影响;最后考察Caspase抑制剂z-VAD-fmk,对trans C18∶1对内皮细胞存活率的影响。吉姆萨染色后,细胞呈典型的凋亡形态;流失细胞仪检测内皮凋亡细胞的数量明显增加;同时发现trans C18∶1引起内皮细胞的凋亡可能与Caspase-3酶激活有关,因为Caspase抑制剂又能提高trans C18∶1处理的内皮细胞存活率。The target of this study is to exoplore the apoptotic mechanism of endothelial cells through detecting relevanting apoptosis index.For this,EC were cultured with trans C18∶1 at different concentrations,then the morphological changes of endothelial cells were observed through giemsa staining.The apoptotic cells were detected by flow cytometry.The activity of caspase-3 was detected by spectrophotography after endothelial cells treated with trans C18∶1.The cell viability was measured by MTT after endothelial cells cultured with trans C18∶1 and caspase inhibitor for 24～48 h.Results： The number of apoptotic cells raised and endothelial cells displayed classical apoptotic morphology after endothelial cells cultured with trans C18∶1.The activity of caspase-3 ascended and the caspase inhibitor could protect endothelial cells from trans C18∶1.

关 键 词：反式脂肪酸 TRANS C18∶1 内皮细胞 凋亡 

分 类 号：R151[医药卫生—营养与食品卫生学]