甲壳素固定化β-葡萄糖醛酸苷酶的研究  

Study on immobilization of β-glucuronidase by chitin

作　　者：孙倩[1] 李开雄[1] 李春[2] 段海燕[2] 叶海[2] 曹红[2] 

机构地区：[1]石河子大学食品学院,新疆石河子832000 [2]石河子大学化学化工学院/化工绿色过程新疆兵团重点实验室,新疆石河子832000

出　　处：《食品工业科技》2011年第9期267-269,共3页Science and Technology of Food Industry

基　　金：教育部科学技术研究重点项目(209146);兵团博士资金项目(2009JC13);2008年度石河子大学高层次人才科研启动资金专项(RCZX200805)

摘　　要：以甲壳素为载体、戊二醛为交联剂,固定β-葡萄糖醛酸苷酶,对β-葡萄糖醛酸苷酶的固定化条件进行了研究,并通过正交实验确定了酶固定的最佳条件:戊二醛浓度0.7%,交联时间4h,吸附时间为9h,反应温度40℃,此时酶活力回收率可达67.32%。The method of immobilized β-glucuronidase was studied in this paper,β-glucuronidase has been immobilized on chitin with glutaraldehyde as crosslinking agent.Some factors that had effect on activity of immobilized β-glucuronidase were studied.The optimal conditions of immobilization were achieved by using the method of orthogonal test,when glutaraldehyde concentration was 0.7%,cross-linking time was 4h,adsorption time was 9h and reaction temperature was 40℃,the recovery rate of immobilized β-glucuronidase activity was 67.32%.

关 键 词：Β-葡萄糖醛酸苷酶 甲壳素 固定化 

分 类 号：TS201.23[轻工技术与工程—食品科学]