重离子诱变对木聚糖酶产生菌产酶的影响  

Studies on the Effects of Heavy-ion Irradiation Xylanase by Streptomyces sp.

作　　者：冯佳丽[1] 台喜生[1] 李梅[1] 李师翁[1] 

机构地区：[1]兰州交通大学化学与生物工程学院,甘肃兰州730070

出　　处：《食品与发酵工业》2011年第8期117-121,共5页Food and Fermentation Industries

基　　金：国家自然科学基金项目(30960063)

摘　　要：以1株由青藏高原牦牛粪中分离出的链霉菌为出发菌株,该菌株在发酵培养基中能产生胞外木聚糖酶(3 227.346 U/mL)。以此菌株为出发菌株,对其进行重离子辐照诱变处理,从大量突变株中筛选出木聚糖酶高产菌株SZ10-7,其酶活力达到5 338.42 U/mL,与出发菌株相比较,突变株SZ10-7的酶活力提高了1.65倍。对突变株SZ10-7的发酵条件进行了优化研究,结果表明,该菌株的木聚糖酶活力得到进一步提高,达到5 850.20U/mL,其最适发酵条件为:培养基(g/100 mL)为玉米芯∶麸皮(体积比1∶1)5,酵母膏0.8,K2 HPO4.3H2 O 0.1,MgSO4.7H20 0.5,NaCl 0.3,pH 7.0,培养温度25℃振荡培养时间96 h,实验结果表明,重离子辐照诱变技术是一种有效的微生物诱变育种新技术。The growth property of Streptomyces sp. , a xylanase producing strain, isolated from the yak dung in Qinghai-Tibet plateau and the nature of xylanase were studied in this paper. Growing in fermentative medium, the strain can produce xylanase （3227. 346U/mL）. We deh with the original strain by the heavy-ion irradiation. We screened a high-yield xylanase producing strain SZ10-7 from many of mutants and its enzyme activity reached to 5 338.42 U/mL. Compared with the original strain, the enzyme activity of mutant strain SZ10-7 increased by 1.65 times. The fermentation conditions of the mutant strain SZ10-7 were optimized and its enzyme activity further increased, reaching to 5850.20 U/mL. The mutant＇s optimum conditions for enzyme production consisted of 5% the mixture of the corn cobs and the wheat bran （1：1）, 0.8% yeast extract, 0.1% K2HPO4 ·3H2O, 0.5% MgSO4 · 7H20,0.3% NaCl, the initial pH 7.0, shaking culture for 96h, at 25℃. These results showed that the heavy-ion irradiation is an effective method for microbe mutagenesis.

关 键 词：重离子诱变 木聚糖酶 发酵 

分 类 号：TQ925[轻工技术与工程—发酵工程]